在蜉蝣目石蝇科的无翅蜉蝣 Ameletus inopinatus 中分离和鉴定 10 个高度多态性的二核苷酸和三核苷酸微卫星标记。

Isolation and characterization of 10 highly polymorphic di- and trinucleotide microsatellite markers in the mayfly Ameletus inopinatus (Ephemeroptera: Siphlonuridae).

机构信息

Research Institute Senckenberg, Department of Limnology and Conservation, Clamecystrasse 12, 63571 Gelnhausen, Germany, Pritzker Laboratory for Molecular Systematics and Evolution, The Field Museum, 1400 S. Lake Shore Drive, Chicago, IL 60605-2496, USA, Institute of Zoology, Department of Molecular Ecology, Johannes Gutenberg University, J.J. Becherweg 13, 55128 Mainz, Germany.

出版信息

Mol Ecol Resour. 2008 Nov;8(6):1285-7. doi: 10.1111/j.1755-0998.2008.02356.x.

DOI:10.1111/j.1755-0998.2008.02356.x

PMID:21586022

Abstract

We describe the isolation of ten polymorphic microsatellite loci from the mayfly Ameletus inopinatus. Loci had di- or trinucleotide repeat motifs and were highly variable with three to 17 alleles (mean = 7.15). Observed heterozygosity ranged from 0.143 to 0.905. One locus (Ami_202) showed significant deviation from Hardy-Weinberg equilibrium in one population, but no evidence for null alleles. One locus (Ami_73) was significantly linked with three other loci. The remaining nine loci should prove highly informative for population genetic studies.

摘要

我们描述了从蜉蝣目 Ameletus inopinatus 中分离出的十个多态性微卫星基因座。这些基因座具有二或三核苷酸重复基序，高度可变，具有三到 17 个等位基因（平均值为 7.15）。观测杂合度范围从 0.143 到 0.905。一个基因座（Ami_202）在一个种群中表现出与哈迪-温伯格平衡显著偏离，但没有无效等位基因的证据。一个基因座（Ami_73）与其他三个基因座显著连锁。其余九个基因座应证明对种群遗传研究具有高度信息性。