10 个二核苷酸和三核苷酸微卫星位点在加勒比刺龙虾 Panulirus argus 中的应用，用于研究区域种群连通性。

Ten di- and trinucleotide microsatellite loci in the Caribbean spiny lobster, Panulirus argus, for studies of regional population connectivity.

机构信息

Florida Fish and Wildlife Research Institute, 100 Eighth Avenue S.E. Saint Petersburg, FL 33701-5095, USA.

出版信息

Mol Ecol Resour. 2008 May;8(3):650-2. doi: 10.1111/j.1471-8286.2007.02032.x.

DOI:10.1111/j.1471-8286.2007.02032.x

Abstract

We describe 10 microsatellite loci for Panulirus argus (Caribbean spiny lobster). The number of alleles at each locus ranged from four to 39 (mean = 21.8) in 89 juvenile specimens collected at two different times at a recruitment site in south Florida. Levels of expected and observed heterozygosities ranged from 0.48 to 0.96 (mean = 0.83) and from 0.32 to 0.98 (mean = 0.71), respectively. Significant departures from Hardy-Weinberg equilibrium were observed at two loci. There was no evidence of genotypic disequilibrium for any pair of loci. Overall, the loci were well resolved, highly polymorphic and independently segregating, confirming their utility for population genetic studies.

摘要

我们为加勒比棘腹龙虾（Panulirus argus）描述了 10 个微卫星位点。在佛罗里达州南部一个繁殖地分两个不同时间采集的 89 只幼体样本中，每个位点的等位基因数范围为 4 到 39（平均值为 21.8）。预期和观察到的杂合度水平范围分别为 0.48 到 0.96（平均值为 0.83）和 0.32 到 0.98（平均值为 0.71）。在两个位点观察到与 Hardy-Weinberg 平衡的显著偏离。没有任何一对位点存在基因型不平衡的证据。总体而言，这些位点分辨率良好，高度多态性且独立分离，证实了它们在种群遗传研究中的实用性。