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从石鳖(Cellana grata)中分离和表征微卫星标记。

Isolation and characterization of microsatellite markers from the limpet Cellana grata.

机构信息

The Swire Institute of Marine Science and Division of Ecology & Biodiversity, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China.

出版信息

Mol Ecol Resour. 2009 May;9(3):902-4. doi: 10.1111/j.1755-0998.2008.02418.x. Epub 2009 Feb 11.

DOI:10.1111/j.1755-0998.2008.02418.x
PMID:21564784
Abstract

This note describes the development of nine polymorphic microsatellite loci in the limpet Cellana grata to investigate population structure and cohort variation in this species. The number of alleles ranged from seven to 22 and observed heterozygosity ranged from 0.62 to 0.95. Deviation from the Hardy-Weinberg equilibrium was detected in two loci, both as a result of heterozygote deficiency. Null alleles were detected in one of these loci. These genetic markers will be used to investigate the genetic structure of C. grata populations, as well as variation among cohorts of this common intertidal species.

摘要

本研究描述了在石鳖科石鳖属的圆石鳖(Cellana grata)中开发的九个多态微卫星标记,以研究该物种的种群结构和群体变异。等位基因数范围为 7 到 22 个,观测杂合度范围为 0.62 到 0.95。两个位点偏离哈迪-温伯格平衡,均为杂合子缺失所致。在其中一个位点检测到无效等位基因。这些遗传标记将用于研究圆石鳖种群的遗传结构,以及这种常见潮间带物种群体间的变异。

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