Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
Mol Ecol Resour. 2008 Nov;8(6):1303-6. doi: 10.1111/j.1755-0998.2008.02137.x.
Microsatellite markers were developed from a fosmid library of female half-smooth tongue sole, Cynoglossus semilaevis. Three hundred eighty-four clones were randomly selected to sequence (double strand reading), and 168 sequences in 143 clones were found to contain microsatellites. Of the 101 primer pairs designed, 64 gave polymorphic polymerase chain reaction products. Based on characterization with 36 individuals, the number of alleles ranged from two to nine. The values of observed and expected heterozygosities varied from 0.06 to 1.00 and from 0.05 to 0.94, respectively. These markers have the potential as tools for population structure evaluation, ecological analyses and linkage map construction.
微卫星标记是从半滑舌鳎（Cynoglossus semilaevis）的雌性部分平滑舌鳎的 fosmid 文库中开发出来的。随机选择了 384 个克隆进行测序（双链读取），在 143 个克隆中的 168 个序列中发现了微卫星。在设计的 101 对引物中，有 64 对产生了多态性聚合酶链反应产物。通过对 36 个个体的特征分析，等位基因数从 2 到 9 不等。观察到的和预期的杂合度值分别从 0.06 到 1.00 和从 0.05 到 0.94 不等。这些标记有可能成为评估种群结构、生态分析和连锁图谱构建的工具。
