Department of Forest Genetics, Forestry and Forest Products Research Institute, Matsunosato, Tsukuba, Ibaraki 305-8687, Japan.
Mol Ecol Resour. 2008 Nov;8(6):1316-8. doi: 10.1111/j.1755-0998.2008.02273.x.
Polymorphic microsatellite markers were developed for Hibiscus glaber, an endemic tree of the Bonin Islands. Eighty-seven of the 208 sequences from an enriched library were unique and containing microsatellites. Ten loci were proved to be highly polymorphic among 78 individuals from the Nishi-jima Island. Total exclusionary powers for the first and the second parents were 99.989% and 99.999%, respectively. Nine loci also amplified single fragment from genomic DNA of H. tiliaceus, a related and widespread congener. Our markers can be reliably used for the estimation of current gene flow within/among populations of the two woody Hibiscus species.
为 Bonin 群岛特有的木槿属植物 Hibiscus glaber 开发了多态性微卫星标记。从富集文库的 208 个序列中，有 87 个是独特的，包含微卫星。在来自西之岛的 78 个个体中，10 个位点被证明是高度多态的。第一和第二亲本的完全排斥力分别为 99.989%和 99.999%。9 个位点也从相关且广泛分布的近缘种黄槿的基因组 DNA 中扩增出单一片段。我们的标记可以可靠地用于估计这两个木槿属物种的种群内/种群间的当前基因流。
