开发柏拉木（柏拉木科）EST-SSR 标记，柏拉木是小笠原群岛的特有种。
Development of EST-SSR markers for Elaeocarpus photiniifolia (Elaeocarpaceae), an endemic taxon of the Bonin Islands.
机构信息
Makino Herbarium, Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo, 192-0397, Japan.
出版信息
Am J Bot. 2012 Feb;99(2):e84-7. doi: 10.3732/ajb.1100391. Epub 2012 Jan 26.
PREMISE OF THE STUDY
Expressed sequence tag (EST)-derived microsatellite markers were developed for Elaeocarpus photiniifolia, an endemic taxon of the Bonin Islands.
METHODS AND RESULTS
Initially, a complementary DNA (cDNA) library was constructed by de novo pyrosequencing of total RNA extracted from a seedling. A total of 267 primer pairs were designed from the library. Of the 48 tested loci, 25 loci were polymorphic among 41 individuals representing the entire geographical range of the species, with the number of alleles per locus and expected heterozygosity ranging from two to 14 and 0.09 to 0.86, respectively. Most loci were transferable to a related species, E. sylvestris.
CONCLUSIONS
The developed markers will be useful for evaluating the genetic structure of E. photiniifolia.
研究前提
从 Bonin 群岛特有种琴叶拟海桑（ Elaeocarpus photiniifolius ）的总 RNA 经从头焦磷酸测序构建 cDNA 文库，开发出基于表达序列标签（EST）的微卫星标记。
方法和结果
最初，通过从头焦磷酸测序构建了一个 cDNA 文库。从文库中设计了 267 对引物。在代表该物种全部地理分布范围的 41 个个体的 48 个测试位点中，有 25 个位点具有多态性，每个位点的等位基因数和预期杂合度分别为 2 至 14 和 0.09 至 0.86。大多数位点可转移到相关物种琴叶拟海桑（E. sylvestris）。
结论
开发的标记将有助于评估琴叶拟海桑的遗传结构。
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