Makino Herbarium, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan, Graduate School of International Development and Cooperation, Hiroshima University, Kagamiyama 1-5-1, Higashi-Hiroshima 739-8529, Japan, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.
Mol Ecol Resour. 2008 Nov;8(6):1423-5. doi: 10.1111/j.1755-0998.2008.02194.x. Epub 2008 Aug 20.
Ten microsatellite loci were isolated and characterized for Callicarpa subpubescens (Verbenaceae), an endemic tree species of the Bonin Islands. The observed number of alleles at each locus ranged from two to eight with an average of 4.9, and the expected heterozygosity ranged from 0.238 to 0.690 with an average of 0.483. All 10 loci were screened in cross-amplification tests for two other endemic Callicarpa species that also inhabit the Bonin Islands. All loci were successfully amplified in these species.
从波恩群岛特有树种驳骨丹(马鞭草科)中分离并鉴定了 10 个微卫星位点。每个位点观察到的等位基因数从 2 到 8 不等,平均值为 4.9,预期杂合度从 0.238 到 0.690 不等,平均值为 0.483。在交叉扩增试验中,对另外两种也栖息在波恩群岛的波恩群岛特有驳骨丹物种进行了 10 个位点的筛选。所有位点在这些物种中均成功扩增。