为卡罗莱纳铁杉（Tsuga caroliniana）分离和表征微卫星标记。

Isolation and characterization of microsatellite markers for Carolina hemlock (Tsuga caroliniana).

机构信息

US Department of Agriculture Forest Service, Southern Research Station, Southern Institute of Forest Genetics, 23332 Mississippi 67, Saucier, MS 39574, USA, North Carolina State University, 3041 Cornwallis Road, Research Triangle Park, NC 27709, USA.

出版信息

Mol Ecol Resour. 2008 Nov;8(6):1371-4. doi: 10.1111/j.1755-0998.2008.02294.x. Epub 2008 Sep 3.

DOI:10.1111/j.1755-0998.2008.02294.x

PMID:21586049

Abstract

We describe the isolation and characterization of 31 polymorphic di- and trinucleotide microsatellite marker loci for Carolina hemlock (Tsuga caroliniana Englem.). In addition, primer pairs for 16 loci amplified scoreable alleles in six other Tsuga species. In eastern North America, both Carolina hemlock and eastern hemlock (Tsuga canadensis [L.] Carr.) populations are declining due to infestation by hemlock woolly adelgid, Adelges tsugae. The markers described here should enhance population genetic studies of hemlocks, providing valuable information for conserving and restoring these important forest tree species.

摘要

我们描述了 31 个多态二核苷酸和三核苷酸微卫星标记位点的分离和特征，这些标记位点用于卡罗莱纳铁杉（Tsuga caroliniana Englem.）。此外，16 个位点的引物对在其他 6 个 Tsuga 物种中扩增出了可评分的等位基因。在北美东部，由于铁杉绵蚜 Adelges tsugae 的侵害，卡罗莱纳铁杉和东部铁杉（Tsuga canadensis [L.] Carr.）种群都在减少。这里描述的标记物应该会增强对铁杉的种群遗传研究，为保护和恢复这些重要的森林树种提供有价值的信息。