从鸸鹋（Dromaius novaehollandiae）序列数据库中开发的新微卫星标记的特征。

Characterization of new microsatellite markers derived from sequence databases for the emu (Dromaius novaehollandiae).

机构信息

Animal Breeding and Genomics Laboratory, Universidad de Chile, Santa Rosa 11735, Santiago, Chile, Laboratory of Molecular Ecology and Evolutionary Studies, Universidad de Santiago de Chile, Avenida Libertador Bernardo O'Higgins 3363, Santiago, Chile, Nutrition, Diabetes, and Metabolism Department, Pontificia Universidad Católica de Chile, Marcoleta 367, Santiago, Chile.

出版信息

Mol Ecol Resour. 2008 Nov;8(6):1442-4. doi: 10.1111/j.1755-0998.2008.02315.x. Epub 2008 Sep 2.

DOI:10.1111/j.1755-0998.2008.02315.x

PMID:21586070

Abstract

The emu (Dromaius novaehollandiae), a member of ratite family, is native to Australia and has been introduced to other countries worldwide. In this work, 10 polymorphic microsatellite loci were isolated and characterized for emu from public sequences. Polymorphism was surveyed in 22 individuals from two different populations kept in captivity. Between two and 11 alleles were found per locus, and the observed heterozygosity ranged from 0.05 to 0.85, in accordance with expectations. These markers will be useful as tools for detecting levels of genetic variation, reconstructing pedigrees (for quantitative genetic analysis) and identifying markers associated to fitness traits in emu populations.

摘要

鸸鹋（Dromaius novaehollandiae），平胸总目鸵鸟目鸸鹋科动物，原产于澳大利亚，现已被引入世界各地的其他国家。本研究从公共序列中分离并鉴定了 10 个鸸鹋微卫星多态性位点。在两个圈养种群的 22 只个体中进行了多态性调查。每个位点发现 2 到 11 个等位基因，观测杂合度范围为 0.05 至 0.85，符合预期。这些标记物将可用于检测遗传变异水平、构建系谱（用于数量遗传分析）以及鉴定与鸸鹋种群适应性特征相关的标记物。