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开发和鉴定薹草(薹草科)微卫星标记。

Development and characterization of microsatellite markers for Polygonum cespitosum (Polygonaceae).

机构信息

Biology Department, Wesleyan University, Middletown, Connecticut 06459, USA.

出版信息

Am J Bot. 2011 Jul;98(7):e180-2. doi: 10.3732/ajb.1100053. Epub 2011 Jun 23.

Abstract

PREMISE OF THE STUDY

We isolated and characterized microsatellite markers in Polygonum cespitosum Blume, an herbaceous annual plant species introduced into North America from Asia that has recently become invasive.

METHODS AND RESULTS

A total of 12 polymorphic and 3 monomorphic loci were screened in 1-2 individuals from each of 20 populations from the introduced and native range, for a total of 24 samples. The number of alleles per locus in the polymorphic loci ranged from 3 to 9, and expected heterozygosity ranged from 0.156 to 0.838.

CONCLUSIONS

These new loci will provide tools for examining genetic relatedness among introduced and native populations of this and other related species.

摘要

研究前提

本研究从亚洲引入北美的一年生草本植物多穗蓼(Polygonum cespitosum Blume),最近已成为入侵物种,我们对其进行了分离和微卫星标记的特征描述。

方法和结果

在引入和本地范围内的 20 个种群中,每个种群随机选取 1-2 个个体进行筛选,共获得 24 个样本。多态性位点的每个位点的等位基因数从 3 到 9 不等,预期杂合度从 0.156 到 0.838 不等。

结论

这些新的标记位点将为研究该物种和其他相关物种的引入种群和本地种群之间的遗传关系提供工具。

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