State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China, Graduate School of the Chinese Academy of Sciences, Beijing 100039, China, Department of Botany, National Museum of Natural History, Smithsonian Institution, Washington D.C. 20013-7012, USA.
Mol Ecol Resour. 2008 Nov;8(6):1491-3. doi: 10.1111/j.1755-0998.2008.02233.x.
We isolated eight novel polymorphic microsatellite loci from Pteridium aquilinum. These loci were characterized in 30 individuals, one from Bolivia, two from Peru, one from the USA, one from Japan, and 25 from Northeast China to Southwest China. The number of alleles per locus ranged from two to seven. The observed heterozygosity (H(O) ) ranged from 0.000 to 0.600 with an average of 0.3051, and the expected heterozygosity (H(E) ) ranged from 0.0966 to 0.7780 with an average of 0.4267. One locus deviated from Hardy-Weinberg equilibrium and four pairs of loci were found to be in linkage disequilibrium. These polymorphic loci will be useful in the study of the population genetic structure of Pteridium.
我们从蕨菜中分离出了 8 个新的多态微卫星位点。这些位点在 30 个个体中进行了特征描述，其中一个来自玻利维亚，两个来自秘鲁，一个来自美国，一个来自日本，25 个来自中国东北到西南。每个位点的等位基因数从 2 到 7 不等。观察到的杂合度（H(O)）范围从 0.000 到 0.600，平均值为 0.3051，预期杂合度（H(E)）范围从 0.0966 到 0.7780，平均值为 0.4267。一个位点偏离哈迪-温伯格平衡，四个对位点存在连锁不平衡。这些多态性位点将有助于研究蕨菜的种群遗传结构。
