长指蝠 Miniopterus fuliginosus 微卫星位点的分离与鉴定。

Isolation and characterization of microsatellite loci in the long-fingered bat Miniopterus fuliginosus.

机构信息

School of Life Science, East China Normal University, Shanghai 200062, China, School of Geographical and Biological Sciences, Guizhou Normal University, Guiyang 550001, China, Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, 130 De Soto Street, Pittsburgh, PA 15261,USA.

出版信息

Mol Ecol Resour. 2008 Jul;8(4):799-801. doi: 10.1111/j.1755-0998.2008.02068.x.

DOI:10.1111/j.1755-0998.2008.02068.x

PMID:21585895

Abstract

We isolated and characterized 10 microsatellite loci in the long-fingered bat Miniopterus fuliginosus. These loci were tested on 48 individuals from Anhui Province of China, and all loci were highly polymorphic. The mean number of observed alleles per locus was 13.6 (range from six to 27). Observed and expected heterozygosity values ranged from 0.364 to 0.957, and from 0.676 to 0.951, respectively. After Bonferroni correction, four loci deviated significantly from Hardy-Weinberg equilibrium. No pairs of loci were in linkage disequilibrium. These polymorphic markers will be used to examine population structure and genetic diversity in this species.

摘要

我们从中国安徽省分离并鉴定了 10 个长指蝠 Miniopterus fuliginosus 的微卫星位点。在 48 个个体中检测了这些位点，所有位点均高度多态。每个位点观察到的等位基因平均数为 13.6（范围为 6 到 27）。观察到的和预期的杂合度值分别为 0.364 到 0.957，以及 0.676 到 0.951。经 Bonferroni 校正后，有 4 个位点偏离 Hardy-Weinberg 平衡显著。没有两个位点处于连锁不平衡状态。这些多态性标记将用于研究该物种的种群结构和遗传多样性。