从黑虎虾（Penaeus monodon）表达序列标签中开发简单重复序列标记。
Development of simple sequence repeat markers from expressed sequence tags of the black tiger shrimp (Penaeus monodon).
机构信息
Institute of Molecular Biology and Genetics, Mahidol University, Salaya, Nakhonpathom 73170, Thailand, National Center for Genetic Engineering and Biotechnology, 113 Phaholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand, Center for Cassava Molecular Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
出版信息
Mol Ecol Resour. 2008 Nov;8(6):1494-6. doi: 10.1111/j.1755-0998.2008.02290.x.
In this study, we describe the development of expressed sequence tag-simple sequence repeat markers from expressed sequence tags of the black tiger shrimp (Penaeus monodon) deposited in public sequence databases. A total of 46 primer pairs were designed and screened on 26 individuals of P. monodon from a natural population. Of these, 16 primer pairs showed polymorphic profiles with between two and five alleles per locus. The average unbiased and direct count heterozygosities were 0.4662 and 0.3516, respectively. Cross-amplification was tested with five individuals of Penaeus vannamei and polymorphic products were detected at five loci.
在这项研究中，我们描述了从公共序列数据库中黑虎虾（Penaeus monodon）的表达序列标签中开发的表达序列标签-简单重复序列标记的发展。总共设计了 46 对引物，并在来自自然种群的 26 个黑虎虾个体上进行了筛选。其中，16 对引物在每个位点显示出具有两个到五个等位基因的多态性图谱。平均无偏和直接计数杂合度分别为 0.4662 和 0.3516。用五个凡纳滨对虾个体进行了交叉扩增，并在五个基因座检测到了多态性产物。
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