Klinbunga Sirawut, Preechaphol Rachanimuk, Thumrungtanakit Supaporn, Leelatanawit Rungnapa, Aoki Takashi, Jarayabhand Padermsak, Menasveta Piamsak
Center of Excellence for Marine Biotechnology, Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Biochem Genet. 2006 Jun;44(5-6):222-36. doi: 10.1007/s10528-006-9027-0. Epub 2006 Sep 23.
A total of 90 ESTs from normal and 157 from subtractive ovarian cDNA libraries of the giant tiger shrimp (Penaeus monodon) were sequenced. SSCP analysis of disulfide isomerase (DSl), zinc finger protein (ZFP), PMO920, and PMT1700 was carried out for population genetic studies of P. monodon in Thai waters. The number of codominant alleles per locus for overall samples was 6 for PMO920, 5 for PMT1700, and 12 for ZFP, and there were 19 dominant alleles for DSI. The observed heterozygosity of each geographic sample was 0.3043-0.5128 for PMO920, 0.3462-0.4643 for PMT1700, and 0.5000-0.8108 for ZFP. Linkage disequilibrium analysis indicated that genotypes of these loci segregate randomly (P > 0.05). Low genetic distance was found between pairs of geographic samples (0.0077-0.0178). The neighbor-joining tree constructed from the average genetic distance of overall loci allocated the Andaman samples (Satun, Trang, and Phangnga) into one cluster, and Chumphon and Trat into other clusters. Geographic differentiation between Satun-Trat and Satun-Phangnga was found only at the ZFP locus (P < 0.05), suggesting low degrees of genetic subdivision of Thai P. monodon.
对来自斑节对虾正常卵巢cDNA文库的90个ESTs和消减卵巢cDNA文库的157个ESTs进行了测序。对泰国水域斑节对虾的群体遗传学研究，进行了二硫键异构酶（DSl）、锌指蛋白（ZFP）、PMO920和PMT1700的SSCP分析。总体样本中，每个位点的共显性等位基因数，PMO920为6个，PMT1700为5个，ZFP为12个，DSI有19个显性等位基因。每个地理样本的观察杂合度，PMO920为0.3043 - 0.5128，PMT1700为0.3462 - 0.4643，ZFP为0.5000 - 0.8108。连锁不平衡分析表明，这些位点的基因型随机分离（P > 0.05）。地理样本对之间的遗传距离较低（0.0077 - 0.0178）。根据所有位点的平均遗传距离构建的邻接树，将安达曼海的样本（沙敦、董里和攀牙）归为一个聚类，春蓬和桐艾归为其他聚类。仅在ZFP位点发现沙敦 - 桐艾和沙敦 - 攀牙之间存在地理分化（P < 0.05），表明泰国斑节对虾的遗传细分程度较低。
