Department of Ecology and Evolutionary Biology, University of California Santa Cruz, 100 Shaffer Road, Santa Cruz, CA 95060, USA.
Mol Ecol Resour. 2008 Nov;8(6):1512-4. doi: 10.1111/j.1755-0998.2008.02346.x.
A set of 12 microsatellite markers was developed from Embiotoca jacksoni genomic DNA and tested for polymorphism using 64 individuals from two populations. All loci were polymorphic with a number of alleles ranging from two to 19 with expected heterozygosities ranging from 0.17 to 0.89. There was no evidence of linkage disequilibrium and all loci were in Hardy-Weinberg equilibrium, except for four loci in one population. High numbers of private alleles were consistent with strong population structure, and very limited dispersal. Six microsatellite markers successfully cross amplified and were polymorphic in closely related species, Embiotoca lateralis and Hyspurus caryi.
从 Embiotoca jacksoni 基因组 DNA 中开发了一套 12 个微卫星标记，并使用来自两个群体的 64 个个体对其多态性进行了测试。所有位点均表现出多态性，等位基因数从 2 到 19 不等，预期杂合度从 0.17 到 0.89 不等。没有连锁不平衡的证据，所有位点均处于哈迪-温伯格平衡状态，除了一个群体中的四个位点。大量的特有等位基因与强烈的种群结构和非常有限的扩散一致。六个微卫星标记在密切相关的物种 Embiotoca lateralis 和 Hyspurus caryi 中成功扩增并具有多态性。
