永久性遗传资源：地中海滨蟹（十足目，梭子蟹科）微卫星位点的分离与特征。

PERMANENT GENETIC RESOURCES: Isolation and characterization of microsatellite loci in the Mediterranean shore crab Carcinus aestuarii (Decapoda, Portunidae).

机构信息

Department of Biology, University of Padova, via U. Bassi 58/B, I-35121 Padova, Italy.

出版信息

Mol Ecol Resour. 2008 Mar;8(2):370-2. doi: 10.1111/j.1471-8286.2007.01960.x.

DOI:10.1111/j.1471-8286.2007.01960.x

Abstract

We characterized nine polymorphic microsatellites in the Mediterranean shore crab Carcinus aestuarii (Decapoda: Portunidae). Microsatellites were isolated from a partial genomic library enriched for multiple motifs. All loci were polymorphic, with number of alleles ranging from two to 16 and a mean observed heterozygosity of 0.75. Seven loci were in Hardy-Weinberg equilibrium, and two showed weak heterozygote deficiency. No linkage disequilibrium was found between loci. In addition, we tested Hardy-Weinberg and linkage equilibrium of three Carcinus maenas loci, already reported to cross-amplify in C. aestuarii. These molecular markers will be potentially useful to investigate genetic structure of this species.

摘要

我们对地中海滨蟹（Carcinus aestuarii）（十足目：梭子蟹科）中的九个多态性微卫星进行了特征描述。微卫星是从富含多种基序的部分基因组文库中分离出来的。所有位点均表现出多态性，等位基因数从 2 到 16 不等，平均观测杂合度为 0.75。7 个位点处于哈迪-温伯格平衡状态，2 个位点表现出微弱的杂合子缺失。在这些位点之间没有发现连锁不平衡。此外，我们还检测了 Carcinus maenas 的三个已报道在 C. aestuarii 中能够交叉扩增的位点的哈迪-温伯格平衡和连锁平衡。这些分子标记可能对研究该物种的遗传结构具有重要意义。