中文：在粉红鲍（Haliotis corrugata）中分离和交叉扩增微卫星。

Isolation and cross-amplification of microsatellites in pink abalone (Haliotis corrugata).

机构信息

Aquaculture Genetics Laboratory, Centro de Investigaciones Biológicas del Noroeste S.C., Mar Bermejo 195 Col. Playa Palo de Santa Rita, La Paz, B.C.S. 23090, México, Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0202, USA.

出版信息

Mol Ecol Resour. 2008 May;8(3):701-3. doi: 10.1111/j.1471-8286.2007.02054.x.

DOI:10.1111/j.1471-8286.2007.02054.x

PMID:21585876

Abstract

Ten novel microsatellite loci were isolated in pink abalone, Haliotis corrugata, using (GT)(15) and (CT)(15 ) enriched genomic libraries. Two previously reported Haliotis kamtschatkana microsatellites cross-amplified in H. corrugata. A set of 12 polymorphic microsatellites were evaluated in a wild population sample (N = 49). The number of alleles ranged from two to 55, and the observed and expected heterozygosities ranged from 0.104 to 0.939 and from 0.213 to 0.982, respectively. Significant deviations from Hardy-Weinberg equilibrium at three loci and no linkage disequilibrium were observed. Haliotis corrugata microsatellites cross-amplified in other abalone species, two in H. fulgens, and seven in H. rufescens.

摘要

利用（GT）（15）和（CT）（15）富集基因组文库，从红鲍（Haliotis corrugata）中分离出 10 个新的微卫星位点。两个先前报道的红鲍（Haliotis kamtschatkana）微卫星在红鲍（H. corrugata）中发生交叉扩增。在一个野生种群样本（N=49）中评估了一组 12 个多态性微卫星。等位基因数范围为 2 到 55，观察到的和预期的杂合度分别为 0.104 到 0.939 和 0.213 到 0.982。在三个位点观察到与哈迪-温伯格平衡的显著偏离，且不存在连锁不平衡。红鲍微卫星在其他鲍物种中发生交叉扩增，在红鲍（H. fulgens）中扩增出两个，在红鲍（H. rufescens）中扩增出七个。