苜蓿中华根瘤菌巨型质粒pRmeSU47b的遗传图谱。
Genetic map of Rhizobium meliloti megaplasmid pRmeSU47b.
作者信息
Charles T C, Finan T M
机构信息
Department of Biology, McMaster University, Hamilton, Ontario, Canada.
出版信息
J Bacteriol. 1990 May;172(5):2469-76. doi: 10.1128/jb.172.5.2469-2476.1990.
Abstract
A circular linkage map of the Rhizobium meliloti megaplasmid pRmeSU47b was constructed. The map consists of transposon insertions carrying alternating antibiotic resistance markers linked by phi M12 transduction. Data from conjugation experiments utilizing donor strains carrying Tn5-oriT insertions in the megaplasmid supported the proposed genetic map. In addition, the positions of previously identified Fix, exopolysaccharide synthetic, thiamine synthetic, and C4-dicarboxylate transport loci on the megaplasmid map were determined. By converting cotransduction frequencies to physical distance, we calculated the replicon to be 1,600 kilobases in size, which compares favorably with previous physical estimates.
摘要
构建了苜蓿根瘤菌大质粒pRmeSU47b的环状连锁图谱。该图谱由携带交替抗生素抗性标记的转座子插入片段组成，这些片段通过φM12转导相连。利用携带大质粒中Tn5 - oriT插入片段的供体菌株进行的接合实验数据支持了所提出的遗传图谱。此外，还确定了大质粒图谱上先前鉴定的固氮（Fix）、胞外多糖合成、硫胺素合成和C4 - 二羧酸转运基因座的位置。通过将共转导频率转换为物理距离，我们计算出复制子大小为1600千碱基，这与先前的物理估计结果相符。
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