枯草杆菌肽酶A同工酶系统。与血清成分的相互作用及其对定量免疫电泳的重要性。

Biochem J. 1975 Oct;151(1):149-55. doi: 10.1042/bj1510149.

A method was developed which involved electroimmunoassay and crossed immunoelectrophoresis of subtilopeptidase A (EC 3.4.21.14). Initial trials with unfractionated antiserum were not successful and interaction of the enzyme with non-immunoglobulin serum components were shown to be the cause of the failures. Quantitative immunoelectrophoresis was possible when purified immunoglobulins were used. A pH of 6.5 (lower than the usual pH 8.6) was necessary to obtain a proper baseline definition. Subtilopeptidase A was confirmed as a multiple isoenzyme system. Qualitative inter-batch variations were detected. Di-isopropyl phosphorofluoridate inhibition altered the electrophoretic pattern, but no loss of antigenic determinants was observed.

开发了一种涉及枯草杆菌肽酶A（EC 3.4.21.14）的免疫电泳和交叉免疫电泳的方法。使用未分级抗血清的初步试验未成功，结果表明酶与非免疫球蛋白血清成分的相互作用是失败的原因。使用纯化的免疫球蛋白时可以进行定量免疫电泳。为了获得合适的基线清晰度，需要pH值为6.5（低于通常的pH 8.6）。枯草杆菌肽酶A被确认为多同工酶系统。检测到批次间的定性差异。二异丙基氟磷酸酯抑制改变了电泳图谱，但未观察到抗原决定簇的丢失。