Iemura K, Takebayashi Y, Miyadera K, Yamada Y, Akiyama S, Nagata Y
KAGOSHIMA UNIV,CANC RES INST,DEPT CANC CHEMOTHERAPY,KAGOSHIMA 890,JAPAN. KAGOSHIMA UNIV,FAC MED,DEPT OBSTET & GYNECOL,KAGOSHIMA 890,JAPAN. TAISHO PHARMACEUT CO LTD,SAITAMA 357,JAPAN.
Oncol Rep. 1997 Nov-Dec;4(6):1181-5. doi: 10.3892/or.4.6.1181.
Thymidine phosphorylase (dThdPase) is an enzyme involved in pyrimidine nucleoside metabolism. dThdPase activity is increased in various types of malignant tumors. Recently, we demonstrated that dThdPase is identical to platelet-derived endothelial cell growth factor (PD-ECGF), and has angiogenic activity. We measured the dThdPase activity and the level of thrombomodulin (TM), as a marker for endothelial cells, in ovarian carcinomas, benign tumors and normal ovarian tissues. The average dThdPase activity of ovarian carcinomas (10.86+/-6.98 nmol/100 mu g protein/h) was significantly higher than that of benign tumors (4.66+/-3.91 nmol/100 mu g protein/h) or normal tissues (2.52+/-1.90 nmol/100 mu g protein/h). The expression of dThdPase detected by immunoblot analysis was well correlated with dThdPase activity. In an immunohistochemical study, the expression of dThdPase was more frequently observed in ovarian carcinomas than in benign tumors or normal tissues. dThdPase activity in human ovarian carcinomas was significantly correlated with the expression of TM in human ovarian carcinomas. These findings suggest that dThdPase expression is significantly correlated with angiogenesis in ovarian tumors.
胸苷磷酸化酶（dThdPase）是一种参与嘧啶核苷代谢的酶。dThdPase活性在各种类型的恶性肿瘤中均升高。最近，我们证明dThdPase与血小板衍生的内皮细胞生长因子（PD-ECGF）相同，并且具有血管生成活性。我们测量了卵巢癌、良性肿瘤和正常卵巢组织中的dThdPase活性以及作为内皮细胞标志物的血栓调节蛋白（TM）水平。卵巢癌的平均dThdPase活性（10.86±6.98 nmol/100μg蛋白质/小时）显著高于良性肿瘤（4.66±3.91 nmol/100μg蛋白质/小时）或正常组织（2.52±1.90 nmol/100μg蛋白质/小时）。通过免疫印迹分析检测到的dThdPase表达与dThdPase活性密切相关。在免疫组织化学研究中，卵巢癌中dThdPase的表达比良性肿瘤或正常组织中更常见。人卵巢癌中的dThdPase活性与人卵巢癌中TM的表达显著相关。这些发现表明dThdPase表达与卵巢肿瘤中的血管生成显著相关。
