Chen Wei, Xu Naifeng, Xu Liguang, Wang Libing, Li Zuokun, Ma Wei, Zhu Yingyue, Xu Chuanlai, Kotov Nicholas A
Department of Chemical Engineering, University of Michigan, Ann Arbor, 48109 Michigan, USA; School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, China.
Macromol Rapid Commun. 2010 Jan 18;31(2):228-36. doi: 10.1002/marc.200900793. Epub 2010 Jan 5.
In this work, we describe the preparation and biomedical functionalities of complex nanoparticle assemblies with magnetoplasmonic properties suitable for simultaneous cancer therapy and diagnostics (theranostics). Most commonly magnetoplasmonic nanostructures are made by careful adaptation of metal reduction protocols which is both tedious and restrictive. Here we apply the strategy of nanoscale assemblies to prepare such systems from individual building blocks. The prepared superstructures are based on magnetic Fe(3) O(4) nanoparticles encapsulated in silica shell representing the magnetic module. The cores are surrounded in a corona-like fashion by gold nanoparticles representing the plasmonic module. As additional functionality they were also coated by poly(ethyleneglycol) chains as a cloaking agent to extend the blood circulation time. The preparation is exceptionally simple and allows one to vary the contribution of each function. Both modules can carry drugs and, in this study, they were loaded with the potential anticancer drug curcumin. A comprehensive set of microscopy, spectroscopy and biochemical methods were applied to characterize both imaging and therapeutic function of the nanoparticle assemblies against leukemia HL-60 cells. High contrast magnetic resonance images and high apoptosis rates demonstrate the success of assembly approach for the preparation of magnetoplasmonic nanoparticles. This technology allows one to easily "dial in" the functionalities in the clinical setting for personalized theranostic regiments.
在这项工作中，我们描述了具有磁等离子体特性的复合纳米颗粒组装体的制备及其生物医学功能，这些特性适用于同步癌症治疗和诊断（治疗诊断学）。最常见的磁等离子体纳米结构是通过精心调整金属还原方案制成的，这既繁琐又受限。在这里，我们应用纳米级组装策略，从单个构建块制备此类系统。所制备的超结构基于包裹在二氧化硅壳中的磁性Fe₃O₄纳米颗粒，代表磁性模块。核心被代表等离子体模块的金纳米颗粒以冠状方式包围。作为附加功能，它们还用聚乙二醇链作为隐形剂进行了包覆，以延长血液循环时间。制备过程异常简单，并且允许改变每个功能的贡献。两个模块都可以携带药物，在本研究中，它们装载了潜在的抗癌药物姜黄素。应用了一套全面的显微镜、光谱和生化方法来表征纳米颗粒组装体对白血病HL - 60细胞的成像和治疗功能。高对比度磁共振图像和高凋亡率证明了组装方法在制备磁等离子体纳米颗粒方面的成功。这项技术使人们能够在临床环境中轻松地“调节”功能，以实现个性化的治疗诊断方案。
