人参皂苷 Rh1 和 Rg2 对神经元细胞的神经保护作用。
Neuroprotective effects of ginsenosides Rh1 and Rg2 on neuronal cells.
机构信息
Department of Biology, Faculty of Science, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China.
出版信息
Chin Med. 2011 May 19;6(1):19. doi: 10.1186/1749-8546-6-19.
BACKGROUND
The present study investigates the effects of ginsenosides Rh1 and Rg2 against 6-hydroxydopamine (6-OHDA), a neurotoxin on SH-SY5Y cells and PC-12 cells. The effects of these two ginsenosides on neuronal differentiation are also examined.
METHODS
LDH assay was used to measure cell viability after exposure to 6-OHDA and ginsenosides. Neuronal differentiation was evaluated by changes in cell morphology and density of neurite outgrowths. Western blotting was used to determine the ginsenosides' effects on activation of extracellular signal-regulated protein kinases (ERKs).
RESULTS
Rh1 and Rg2 attenuated 6-OHDA toxicity in SH-SY5Y cells and induced neurite outgrowths in PC-12 cells. 6-OHDA-induced ERK phosphorylation was decreased by Rh1 and Rg2. 20(R)-form and 20(S)-form of the ginsenosides exerted similar effects in inducing neurite outgrowths in PC-12 cells.
CONCLUSION
The present study demonstrates neuroprotective effects of ginsenosides Rh1 and Rg2 on neuronal cell lines. These results suggest potential Chinese medicine treatment for neurodegenerative disorders (eg Parkinson's disease).
背景
本研究探讨了人参皂苷 Rh1 和 Rg2 对神经毒素 6-羟多巴胺（6-OHDA）对 SH-SY5Y 细胞和 PC-12 细胞的作用。还研究了这两种人参皂苷对神经元分化的影响。
方法
通过测定乳酸脱氢酶（LDH）评估细胞活力，检测细胞暴露于 6-OHDA 和人参皂苷后的活力。通过细胞形态和突起生长密度的变化评估神经元分化。采用 Western blot 检测人参皂苷对细胞外信号调节激酶（ERK）激活的影响。
结果
Rh1 和 Rg2 减轻了 6-OHDA 对 SH-SY5Y 细胞的毒性，并诱导了 PC-12 细胞的突起生长。Rh1 和 Rg2 降低了 6-OHDA 诱导的 ERK 磷酸化。20(R)-和 20(S)-形式的人参皂苷对 PC-12 细胞的突起生长具有相似的诱导作用。
结论
本研究证明了人参皂苷 Rh1 和 Rg2 对神经细胞系的神经保护作用。这些结果提示了中药治疗神经退行性疾病（如帕金森病）的潜在可能性。
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