Seoul National University, College of Veterinary Medicine, Department of Veterinary Pathology, 599 Gwanak-ro, Gwanak-Gu, 151-742 Seoul, Republic of Korea.
Res Vet Sci. 2012 Jun;92(3):486-91. doi: 10.1016/j.rvsc.2011.04.016. Epub 2011 May 17.
The expression patterns of different secreted (MUC2, MUC5AC, MUC5B, and MUC6) and membrane-bound (MUC4) mucins were determined immunohistochemically in the lungs of pigs experimentally infected with Actinobacillus pleuropneumoniae. Forty-seven-week-old colostrum-deprived pigs were randomly allocated to infected (n=20) or control groups (n=20). Five infected and uninfected pigs were euthanized at 0, 6, 12, and 48 h post-inoculation (hpi). In the infected pigs, the expression of both types of mucins, which were invariably observed, was associated with bronchiolar and respiratory bronchiolar lesions. Strong positive mucin signals were seen on the surface of bronchiolar and respiratory bronchiolar epithelium with neutrophil infiltration. The mean mucin-positive area peaked at 6 hpi and decreased significantly to control levels by 48 hpi on the surface of the bronchiolar and respiratory bronchiolar epithelium. Further studies are needed to establish the functional relationship between mucin expression and the host defense mechanism against A. pleuropneumoniae in the lungs of infected pigs.
采用免疫组织化学方法检测了实验感染胸膜肺炎放线杆菌的猪肺部不同分泌型（MUC2、MUC5AC、MUC5B 和 MUC6）和膜结合型（MUC4）粘蛋白的表达模式。47 周龄的初乳剥夺猪被随机分配到感染组（n=20）或对照组（n=20）。5 头感染和未感染的猪分别在接种后 0、6、12 和 48 小时（hpi）处死。在感染猪中，两种类型的粘蛋白（始终观察到）的表达与细支气管和呼吸性细支气管病变有关。在细支气管和呼吸性细支气管上皮细胞表面，伴有中性粒细胞浸润，观察到强烈的阳性粘蛋白信号。在细支气管和呼吸性细支气管上皮细胞表面，粘蛋白阳性区域的平均面积在 6 hpi 时达到峰值，并在 48 hpi 时显著下降至对照水平。需要进一步研究以确定粘蛋白表达与感染猪肺部针对胸膜肺炎放线杆菌的宿主防御机制之间的功能关系。
