Oh Y, Ha Y, Han K, Seo H W, Kang I, Park C, Kim S C, Kim S-H, Chae C
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea.
J Comp Pathol. 2013 Feb;148(2-3):259-65. doi: 10.1016/j.jcpa.2012.06.002. Epub 2012 Jul 20.
The aim of this study was to determine the expression of leucocyte function-associated antigen (LFA)-1 (CD11a/CD18) by neutrophils and intercellular adhesion molecule (ICAM)-1 (CD54) by endothelial cells in the lungs of pigs that had been infected experimentally with Actinobacillus pleuropneumoniae. Sixty-four 7-week-old conventional pigs were allocated randomly into infected (n = 40) or control (n = 24) groups. Five infected and three uninfected pigs were killed at 3, 6, 9, 12, 24, 36, 48 and 60 h post inoculation (hpi). Strong immunohistochemical expression of LFA-1 and ICAM-1 was detected frequently in neutrophils in the alveolar space and in endothelial cells in the capillaries of the alveolar septa, respectively. LFA-1 and ICAM-1 expression appeared to correlate with the onset of neutrophil infiltration into the alveolar space. The interaction between ICAM-1 and LFA-1 may be associated with the adherence of neutrophils to vascular endothelium, thereby permitting transmigration of these cells into inflamed lung.
本研究的目的是确定实验感染胸膜肺炎放线杆菌的猪肺中嗜中性粒细胞的白细胞功能相关抗原（LFA）-1（CD11a/CD18）表达以及内皮细胞的细胞间黏附分子（ICAM）-1（CD54）表达。64头7周龄的普通猪被随机分为感染组（n = 40）和对照组（n = 24）。在接种后3、6、9、12、24、36、48和60小时（hpi）处死5头感染猪和3头未感染猪。分别在肺泡腔中的嗜中性粒细胞和肺泡间隔毛细血管中的内皮细胞中频繁检测到LFA-1和ICAM-1的强免疫组化表达。LFA-1和ICAM-1表达似乎与嗜中性粒细胞浸润到肺泡腔的开始相关。ICAM-1和LFA-1之间的相互作用可能与嗜中性粒细胞黏附于血管内皮有关，从而使这些细胞能够迁移到炎症肺组织中。
