Lima Filho J L, Ledingham W M
Departamento de Bioquimica, Universidade Federal de Pernambuco, Brazil.
Appl Biochem Biotechnol. 1990 Feb;23(2):181-6. doi: 10.1007/BF02798386.
The gene (GDH1) coding for the NADP-linked glutamate dehydrogenase system (NADP-GDH) has been cloned from Saccharomyces cerevisiae strain. Cells being transformed by the NADP-GDH gene on a 2 micron bared vector (pCYG4) plasmid confering 11-fold higher level on expressed GDH activity over the wild-type cells. The behavior of these cells was investigated under chemostatic growth with a carbon rate-limiting nutrient. Specific growth rates of cells carrying plasmid pCYG4 were found to be slightly slower than wild type cells. Furthermore, the NADP-GDH activity increases proportionally with the dilution rate. In addition, oscillations in the NADP-GDH activity, especially at a dilution rate up to 0.15/h, are probably consequential on the appearance of a changing mixed population (cells with and without plasmids).
编码与NADP相关的谷氨酸脱氢酶系统（NADP-GDH）的基因（GDH1）已从酿酒酵母菌株中克隆出来。用2微米裸露载体（pCYG4）质粒上的NADP-GDH基因转化的细胞，其表达的GDH活性比野生型细胞高11倍。在以碳源为限制营养物的恒化生长条件下研究了这些细胞的行为。发现携带质粒pCYG4的细胞的比生长速率比野生型细胞略慢。此外，NADP-GDH活性与稀释率成比例增加。另外，NADP-GDH活性的振荡，特别是在稀释率高达0.15/h时，可能是由于出现变化的混合群体（有质粒和无质粒的细胞）所致。
