Lima Filho J L, Ledingham W M
Departamento de Bioquimica, Universidade Federal de Pernambuco, Cidade Universitaria, Recife-PE, Brasil.
Appl Biochem Biotechnol. 1988 Oct;19(1):27-32. doi: 10.1007/BF02921463.
Batch culture experiments of three different strains of Saccharomyces cerevisiae have been carried out. The first strain was transformed by a plasmid pCYG4, which carries the glutamate dehydrogenase (NADP-GDH, E.C. 1.4.14) gene conferring an 11-fold increase in activity. The second was transformed by the same plasmid, but without NADP-GDH, and the third was the wild type. The specific growth rates of the two recombinant DNA strains were below that of the wild type, which can be related to extra plasmid protein production.
已对三种不同的酿酒酵母菌株进行了分批培养实验。第一种菌株用携带谷氨酸脱氢酶（NADP-GDH，E.C. 1.4.14）基因的质粒pCYG4进行转化，该基因使活性提高了11倍。第二种菌株用相同的质粒进行转化，但不含NADP-GDH，第三种是野生型。两种重组DNA菌株的比生长速率低于野生型，这可能与额外的质粒蛋白产生有关。
