Facultad de Veterinaria, Universidad de Murcia, Murcia, Spain.
Vet Parasitol. 2011 Sep 8;181(1):12-6. doi: 10.1016/j.vetpar.2011.04.016. Epub 2011 Apr 19.
An epidemiological study was carried out to investigate asymptomatic Leishmania infantum infection by PCR and ELISA in wild rabbits, humans and domestic dogs in southeastern Spain. Seroprevalence was 0% (0/36) in rabbits, 2% (13/657) in humans and 7% (14/208) in dogs. The prevalence of PCR-positives was 0.6% (1/162) in rabbits tested in a wide range of tissue samples, 2% (8/392) in humans analysed in blood samples and 10% (20/193) and 67% (29/43) in dogs analysed in blood and lymphoid tissue samples, respectively. Results suggest that wild rabbits have a very low risk of becoming chronically infected with L. infantum, and provide further evidence that cryptic L. infantum infection is widespread in the domestic dog population and is also present in a comparatively smaller proportion of healthy humans. The epidemiological and clinical implications of these findings are discussed.
在西班牙东南部，对野生兔、人类和家养犬进行了一项关于 PCR 和 ELISA 检测无症状利什曼原虫感染的流行病学研究。兔的血清阳性率为 0%（0/36），人类为 2%（13/657），犬为 7%（14/208）。在广泛的组织样本中检测到的兔 PCR 阳性率为 0.6%（1/162），血液样本中检测到的人类阳性率为 2%（8/392），血液和淋巴组织样本中检测到的犬阳性率分别为 10%（20/193）和 67%（29/43）。结果表明，野生兔感染利什曼原虫后慢性感染的风险非常低，并进一步证明隐性利什曼原虫感染在犬群中广泛存在，且在健康人群中也存在一定比例。讨论了这些发现的流行病学和临床意义。
