Department of Microbial Biotechnology and Cell Biology, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary.
Bioresour Technol. 2011 Jul;102(14):7232-5. doi: 10.1016/j.biortech.2011.04.080. Epub 2011 Apr 29.
A thermotolerant Kluyveromyces marxianus mutant was developed by exposing yeast cultures repeatedly to 48°C incubation temperature, and the strain was characterized with a significantly increased trehalose content. Unexpectedly, the strain was sensitive to alcohol, osmotic and oxidative stress, which correlated with the increases in the trehalose concentrations. Intracellular glutathione levels declined in both wild-type and mutant cells when exposed to elevating incubation temperatures. Finally, we reached the surprising conclusion that neither trehalose nor glutathione metabolisms should be aimed at in future strain development programs with K. marxianus.
一株耐热克鲁维酵母（Kluyveromyces marxianus）突变株是通过反复暴露于 48°C 培养温度来开发的，该菌株的特征是海藻糖含量显著增加。出乎意料的是，该菌株对酒精、渗透和氧化应激敏感，这与海藻糖浓度的增加有关。当暴露于升高的培养温度时，野生型和突变细胞中的细胞内谷胱甘肽水平下降。最后，我们得出了一个令人惊讶的结论，即在未来的克鲁维酵母（K. marxianus）菌株开发计划中，既不应该针对海藻糖代谢，也不应该针对谷胱甘肽代谢。
