利用氧化葡萄糖酸杆菌在气升式生物反应器中生产 1,3-二羟基丙酮的甘油。

Use of glycerol for producing 1,3-dihydroxyacetone by Gluconobacter oxydans in an airlift bioreactor.

机构信息

Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China.

出版信息

Bioresour Technol. 2011 Jul;102(14):7177-82. doi: 10.1016/j.biortech.2011.04.078. Epub 2011 Apr 29.

DOI:10.1016/j.biortech.2011.04.078

Abstract

1,3-Dihydroxyacetone can be produced by biotransformation of glycerol with glycerol dehydrogenase from Gluconobacter oxydans cells. Firstly, improvement the activity of glycerol dehydrogenase was carried out by medium optimization. The optimal medium for cell cultivation was composed of 5.6g/l yeast extract, 4.7 g/l glycerol, 42.1g/l mannitol, 0.5 g/l K(2)HPO(4), 0.5 g/l KH(2)PO(4), 0.1g/l MgSO(4)·7H(2)O, and 2.0 g/l CaCO(3) with the initial pH of 4.9. Secondly, an internal loop airlift bioreactor was applied for DHA production from glycerol by resting cells of G. oxydans ZJB09113. Furthermore, the effects of pH, aeration rate and cell content on DHA production and glycerol feeding strategy were investigated. 156.3 ± 7.8 g/l of maximal DHA concentration with 89.8±2.4% of conversion rate of glycerol to DHA was achieved after 72h of biotransformation using 10g/l resting cells at 30°C, pH 5.0 and 1.5vvm of aeration rate.

摘要

1,3-二羟基丙酮可以通过氧化葡萄糖酸杆菌细胞中的甘油脱氢酶将甘油进行生物转化来生产。首先，通过培养基优化来提高甘油脱氢酶的活性。细胞培养的最佳培养基由 5.6g/l 酵母提取物、4.7 g/l 甘油、42.1g/l 甘露醇、0.5 g/l K(2)HPO(4)、0.5 g/l KH(2)PO(4)、0.1g/l MgSO(4)·7H(2)O 和 2.0 g/l CaCO(3)组成，初始 pH 值为 4.9。其次，采用内置环流气升式生物反应器，利用氧化葡萄糖酸杆菌 ZJB09113 的休眠细胞从甘油生产 DHA。此外，还考察了 pH 值、通气速率和细胞含量对 DHA 生产和甘油进料策略的影响。在 30°C、pH 值 5.0 和 1.5vvm 的通气速率下，使用 10g/l 休眠细胞进行生物转化 72 小时后，可获得 156.3±7.8g/l 的最大 DHA 浓度和 89.8±2.4%的甘油转化率。