在鼓泡式生物反应器中，通过甘油和尿素进料策略，利用氧化葡萄糖酸杆菌固定化细胞对甘油进行重复生物转化生成 1,3-二羟基丙酮。
Repeated biotransformation of glycerol to 1,3-dihydroxyacetone by immobilized cells of Gluconobacter oxydans with glycerol- and urea-feeding strategy in a bubble column bioreactor.
机构信息
Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
出版信息
Bioresour Technol. 2017 Jun;233:144-149. doi: 10.1016/j.biortech.2017.02.096. Epub 2017 Feb 24.
Some inorganic nitrogen sources and amino acids instead of yeast extract, which resulted in trouble of product purification, were introduced for 1,3-dihydroxyacetone (DHA) production by biotransformation with Gluconobacter oxydans. The results showed that urea is an optimal nitrogen source. Furthermore, the effects of glycerol- and urea-feeding strategies for DHA production by immobilized cells in a home-made bubble column bioreactor were optimized. Cells immobilization was prepared by cultivation in the bioreactor packed with porous ceramics, and then the broth was removed. Then, repeated biotransformation by continuous-feeding of glycerol and urea was developed. Up to 96.4±4.1g/L of average DHA concentration with 94.8±2.2% of average conversion rate of glycerol to DHA was achieved after 12 cycles of run. Near colorless DHA solution with few impurities was obtained and the production cost could be decreased.
一些无机氮源和氨基酸被引入到氧化葡萄糖酸杆菌的生物转化生产 1,3-二羟基丙酮（DHA）中，替代了酵母提取物，这导致了产品纯化的困难。结果表明，尿素是一种最佳的氮源。此外，还优化了在家用鼓泡柱生物反应器中固定化细胞生产 DHA 时甘油和尿素补料策略的效果。细胞固定化是在填充多孔陶瓷的生物反应器中培养后进行的，然后去除发酵液。然后，通过连续补料甘油和尿素进行重复生物转化。经过 12 个循环的运行，可获得 96.4±4.1g/L 的平均 DHA 浓度和 94.8±2.2%的甘油到 DHA 的平均转化率。得到了几乎无色的 DHA 溶液，杂质较少，降低了生产成本。
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