猫冠状病毒感染后家猫全胎（Fcwf - 4）细胞培养的超微结构
Ultrastructure of Felis catus whole fetus (Fcwf-4) cell culture following infection with feline coronavirus.
作者信息
Alazawy Amer, Arshad Siti-Suri, Bejo Mohd-Hair, Omar Abdul-Rahman, Tengku Ibrahim Tengku-Azmi, Sharif Saeed, Bande Faruku, Awang-Isa Kamarudin
机构信息
Department of Veterinary Pathology and Microbiology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
出版信息
J Electron Microsc (Tokyo). 2011;60(4):275-82. doi: 10.1093/jmicro/dfr031. Epub 2011 May 18.
Feline coronavirus (FCoV) consists of two biotypes based on their growth in cell culture and their antigenicity. Infections with FCoV are highly prevalent in the cat population worldwide. In this study, Felis catus whole fetus (Fcwf-4)cell culture was infected with FCoV UPM11C/08. Virus multiplication in cell culture was monitored and examined under the transmission electron microscope. The virus particles revealed the characteristic morphology of feline FCoV represented by envelope viruses surrounded by peplomers. Virus attachment and entry into the cell occurred 15 h post-infection (pi), and the myriad of virus particles were observed both extracellularly and intracellularly after 48 h pi. Thereafter, intracellular virus particles were observed to be present in vacuoles or present freely in the cytoplasm.
猫冠状病毒（FCoV）根据其在细胞培养中的生长情况和抗原性可分为两种生物型。FCoV感染在全球猫群中极为普遍。在本研究中，用FCoV UPM11C/08感染猫全胎（Fcwf-4）细胞培养物。监测细胞培养中的病毒增殖，并在透射电子显微镜下进行检查。病毒颗粒呈现出猫FCoV的特征形态，表现为被纤突包围的包膜病毒。病毒在感染后15小时（pi）附着并进入细胞，在感染后48小时，细胞外和细胞内均观察到大量病毒颗粒。此后，观察到细胞内病毒颗粒存在于液泡中或自由存在于细胞质中。
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