Waters Corporation, MS Technologies Centre, Floats Road, Manchester M23 9LZ, UK.
Rapid Commun Mass Spectrom. 2011 Jun 15;25(11):1559-66. doi: 10.1002/rcm.5013.
The use of ion mobility separation to determine the collision cross-section of a gas-phase ion can provide valuable structural information. The introduction of travelling-wave ion mobility within a quadrupole/time-of-flight mass spectrometer has afforded routine collision cross-section measurements to be performed on a range of ionic species differing in gas-phase size/structure and molecular weight at physiologically relevant concentrations. Herein we discuss the technical advances in the second-generation travelling-wave ion mobility separator, which result in up to a four-fold increase in mobility resolution. This improvement is demonstrated using two reverse peptides (mw 490 Da), small ruthenium-containing anticancer drugs (mw 427 Da), a cisplatin-modified protein (mw 8776 Da) and the noncovalent tetradecameric chaperone complex GroEL (mw 802 kDa). What is also shown are that the collision cross-sections determined using the second-generation mobility separator correlate well with the previous generation and theoretically derived values.
利用离子淌度分离来确定气相离子的碰撞截面可以提供有价值的结构信息。在四极杆/飞行时间质谱仪中引入连续波离子淌度，使得在生理相关浓度下，可以对在气相尺寸/结构和分子量上存在差异的一系列离子物种进行常规的碰撞截面测量。本文讨论了第二代连续波离子淌度分离器的技术进展，该技术使淌度分辨率提高了四倍。这一改进通过使用两个反向肽（mw 490 Da）、两种含钌的小分子抗癌药物（mw 427 Da）、一种顺铂修饰的蛋白质（mw 8776 Da）和非共价十四聚体伴侣复合物 GroEL（mw 802 kDa）进行了演示。结果还表明，使用第二代淌度分离器确定的碰撞截面与前一代和理论推导的值很好地相关。
