Department of Tissue and Organ Development, Regeneration, and Advanced Medical Science, Gifu University Graduate School of Medicine, CREST-JST, Gifu, Japan.
Dev Dyn. 2011 Jul;240(7):1681-93. doi: 10.1002/dvdy.22658. Epub 2011 May 18.
Multipotency of neural crest cells (NC cells) is thought to be a transient phase at the early stage of their generation; after NC cells emerge from the neural tube, they are specified into the lineage-restricted precursors. We analyzed the differentiation of early-stage NC-like cells derived from Sox10-IRES-Venus ES cells, where the expression of Sox10 can be visualized with a fluorescent protein. Unexpectedly, both the Sox10+/Kit- cells and the Sox10+/Kit+ cells, which were restricted in vivo to the neuron (N)-glial cell (G) lineage and melanocyte (M) lineage, respectively, generated N, G, and M, showing that they retain multipotency. We generated mice from the Sox10-IRES-Venus ES cells and analyzed the differentiation of their NC cells. Both the Sox10+/Kit- cells and Sox10+/Kit+ cells isolated from these mice formed colonies containing N, G, and M, showing that they are also multipotent. These findings suggest that NC cells retain multipotency even after the initial lineage-restricted stages.
神经嵴细胞（NC 细胞）的多能性被认为是其早期产生的一个短暂阶段；NC 细胞从神经管中出现后，被特化成为谱系限制前体细胞。我们分析了 Sox10-IRES-Venus ES 细胞中衍生的早期 NC 样细胞的分化，其中 Sox10 的表达可以通过荧光蛋白可视化。出乎意料的是，体内分别限制为神经元（N）-神经胶质细胞（G）谱系和黑色素细胞（M）谱系的 Sox10+/Kit-细胞和 Sox10+/Kit+细胞都产生了 N、G 和 M，表明它们保留了多能性。我们从 Sox10-IRES-Venus ES 细胞中生成了小鼠，并分析了它们的 NC 细胞的分化。从这些小鼠中分离的 Sox10+/Kit-细胞和 Sox10+/Kit+细胞都形成了含有 N、G 和 M 的集落，表明它们也是多能的。这些发现表明，NC 细胞甚至在最初的谱系限制阶段之后仍然保留多能性。
