• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

SOX10 在维持产后黑素细胞谱系和黑素细胞干细胞祖细胞分化中的双重作用。

A dual role for SOX10 in the maintenance of the postnatal melanocyte lineage and the differentiation of melanocyte stem cell progenitors.

机构信息

Genetic Disease Research Branch, National Human Genome Institute, National Institutes of Health, Bethesda, Maryland, United States of America.

出版信息

PLoS Genet. 2013;9(7):e1003644. doi: 10.1371/journal.pgen.1003644. Epub 2013 Jul 25.

DOI:10.1371/journal.pgen.1003644
PMID:23935512
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3723529/
Abstract

During embryogenesis, the transcription factor, Sox10, drives the survival and differentiation of the melanocyte lineage. However, the role that Sox10 plays in postnatal melanocytes is not established. We show in vivo that melanocyte stem cells (McSCs) and more differentiated melanocytes express SOX10 but that McSCs remain undifferentiated. Sox10 knockout (Sox10(fl); Tg(Tyr::CreER)) results in loss of both McSCs and differentiated melanocytes, while overexpression of Sox10 (Tg(DctSox10)) causes premature differentiation and loss of McSCs, leading to hair graying. This suggests that levels of SOX10 are key to normal McSC function and Sox10 must be downregulated for McSC establishment and maintenance. We examined whether the mechanism of Tg(DctSox10) hair graying is through increased expression of Mitf, a target of SOX10, by asking if haploinsufficiency for Mitf (Mitf(vga9) ) can rescue hair graying in Tg(DctSox10) animals. Surprisingly, Mitf(vga9) does not mitigate but exacerbates Tg(DctSox10) hair graying suggesting that MITF participates in the negative regulation of Sox10 in McSCs. These observations demonstrate that while SOX10 is necessary to maintain the postnatal melanocyte lineage it is simultaneously prevented from driving differentiation in the McSCs. This data illustrates how tissue-specific stem cells can arise from lineage-specified precursors through the regulation of the very transcription factors important in defining that lineage.

摘要

在胚胎发生过程中，转录因子 Sox10 驱动黑素细胞谱系的存活和分化。然而， Sox10 在出生后黑素细胞中的作用尚未确定。我们在体内显示黑素细胞干细胞 (McSCs) 和更分化的黑素细胞表达 SOX10 ，但 McSCs 仍未分化。 Sox10 敲除 ( Sox10(fl); Tg(Tyr::CreER)) 导致 McSCs 和分化的黑素细胞丢失，而过表达 Sox10 ( Tg(DctSox10)) 导致过早分化和 McSCs 丢失，导致头发变白。这表明 SOX10 的水平对正常 McSC 功能至关重要，并且 Sox10 必须下调以建立和维持 McSC。我们通过询问 Mitf ( SOX10 的靶标) 的表达是否增加来检查 Tg(DctSox10) 头发变白的机制，Mitf 缺失 ( Mitf(vga9) ) 是否可以挽救 Tg(DctSox10) 动物的头发变白。令人惊讶的是， Mitf(vga9) 并没有减轻但加剧了 Tg(DctSox10) 头发变白，这表明 MITF 参与了 McSCs 中 Sox10 的负调控。这些观察结果表明，虽然 SOX10 对于维持出生后黑素细胞谱系是必需的，但它同时被阻止在 McSCs 中驱动分化。这些数据表明，组织特异性干细胞如何通过调节对定义该谱系非常重要的转录因子，从谱系指定的前体中产生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22e3/3723529/b66ac81448f4/pgen.1003644.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22e3/3723529/d271876283bc/pgen.1003644.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22e3/3723529/310c112d42e3/pgen.1003644.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22e3/3723529/6a6c0ab1e14d/pgen.1003644.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22e3/3723529/9fef77fea4d5/pgen.1003644.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22e3/3723529/0fb6a956d4f6/pgen.1003644.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22e3/3723529/fda2e41ecd83/pgen.1003644.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22e3/3723529/b66ac81448f4/pgen.1003644.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22e3/3723529/d271876283bc/pgen.1003644.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22e3/3723529/310c112d42e3/pgen.1003644.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22e3/3723529/6a6c0ab1e14d/pgen.1003644.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22e3/3723529/9fef77fea4d5/pgen.1003644.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22e3/3723529/0fb6a956d4f6/pgen.1003644.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22e3/3723529/fda2e41ecd83/pgen.1003644.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/22e3/3723529/b66ac81448f4/pgen.1003644.g007.jpg

相似文献

1
A dual role for SOX10 in the maintenance of the postnatal melanocyte lineage and the differentiation of melanocyte stem cell progenitors.SOX10 在维持产后黑素细胞谱系和黑素细胞干细胞祖细胞分化中的双重作用。
PLoS Genet. 2013;9(7):e1003644. doi: 10.1371/journal.pgen.1003644. Epub 2013 Jul 25.
2
An iterative genetic and dynamical modelling approach identifies novel features of the gene regulatory network underlying melanocyte development.一种迭代遗传和动力学建模方法确定了黑素细胞发育相关基因调控网络的新特征。
PLoS Genet. 2011 Sep;7(9):e1002265. doi: 10.1371/journal.pgen.1002265. Epub 2011 Sep 1.
3
CXCL12 regulates differentiation of human immature melanocyte precursors as well as their migration.CXCL12 调节人未成熟黑素细胞前体细胞的分化及其迁移。
Arch Dermatol Res. 2019 Jan;311(1):55-62. doi: 10.1007/s00403-018-1880-2. Epub 2018 Nov 27.
4
A direct link between MITF, innate immunity, and hair graying.MITF、先天免疫与头发变白之间存在直接联系。
PLoS Biol. 2018 May 3;16(5):e2003648. doi: 10.1371/journal.pbio.2003648. eCollection 2018 May.
5
BMP signaling: at the gate between activated melanocyte stem cells and differentiation.BMP 信号转导：在激活的黑素细胞干细胞和分化之间的交界处。
Genes Dev. 2020 Dec 1;34(23-24):1713-1734. doi: 10.1101/gad.340281.120. Epub 2020 Nov 12.
6
Ectopic differentiation of melanocyte stem cells is influenced by genetic background.黑素细胞干细胞的异位分化受遗传背景影响。
Pigment Cell Melanoma Res. 2015 Mar;28(2):223-8. doi: 10.1111/pcmr.12344. Epub 2015 Jan 5.
7
The effects of NB-UVB on the hair follicle-derived neural crest stem cells differentiating into melanocyte lineage in vitro.NB-UVB 对体外毛囊神经嵴干细胞向黑素细胞谱系分化的影响。
J Dermatol Sci. 2012 Apr;66(1):20-8. doi: 10.1016/j.jdermsci.2012.01.012. Epub 2012 Feb 16.
8
Sox10 regulates skin melanocyte proliferation by activating the DNA replication licensing factor MCM5.Sox10通过激活DNA复制许可因子MCM5来调节皮肤黑素细胞的增殖。
J Dermatol Sci. 2017 Mar;85(3):216-225. doi: 10.1016/j.jdermsci.2016.12.002. Epub 2016 Dec 5.
9
B6-Dct-H2BGFP bitransgenic mice: A standardized mouse model for in vivo characterization of melanocyte development and stem cell differentiation.B6-Dct-H2BGFP 双转基因小鼠：一种用于体内鉴定黑素细胞发育和干细胞分化的标准化小鼠模型。
Pigment Cell Melanoma Res. 2021 Sep;34(5):905-917. doi: 10.1111/pcmr.12959. Epub 2021 May 18.
10
The transcription factors Ets1 and Sox10 interact during murine melanocyte development.在小鼠黑素细胞发育过程中，转录因子Ets1和Sox10相互作用。
Dev Biol. 2015 Nov 15;407(2):300-12. doi: 10.1016/j.ydbio.2015.04.012. Epub 2015 Apr 23.

引用本文的文献

1
Melanocyte dysfunctions: future and promise of stem cells.黑素细胞功能障碍：干细胞的未来与前景
Am J Stem Cells. 2025 Jun 15;14(2):25-33. doi: 10.62347/EOIC7075. eCollection 2025.
2
Somatic mutations distinguish melanocyte subpopulations in human skin.体细胞突变可区分人类皮肤中的黑素细胞亚群。
bioRxiv. 2025 Feb 8:2025.02.07.637114. doi: 10.1101/2025.02.07.637114.
3
SASH1 S519N Variant Links Skin Hyperpigmentation and Premature Hair Graying to Dysfunction of Melanocyte Lineage.SASH1 S519N变异将皮肤色素沉着过度和早发性白发与黑素细胞谱系功能障碍联系起来。

本文引用的文献

1
Postnatal lineage mapping of follicular melanocytes with the Tyr::CreER(T) (2) transgene.利用 Tyr::CreER(T) (2) 转基因进行滤泡黑素细胞的产后谱系追踪。
Pigment Cell Melanoma Res. 2013 Mar;26(2):269-74. doi: 10.1111/pcmr.12048. Epub 2012 Dec 20.
2
Sox10 promotes the formation and maintenance of giant congenital naevi and melanoma.Sox10 促进巨大先天性黑素细胞痣和黑色素瘤的形成和维持。
Nat Cell Biol. 2012 Aug;14(8):882-90. doi: 10.1038/ncb2535. Epub 2012 Jul 8.
3
Sox10 controls migration of B16F10 melanoma cells through multiple regulatory target genes.
J Invest Dermatol. 2025 Jan;145(1):144-154.e3. doi: 10.1016/j.jid.2024.04.027. Epub 2024 Jun 6.
4
Melanocyte stem cells in the skin: Origin, biological characteristics, homeostatic maintenance and therapeutic potential.皮肤中的黑素细胞干细胞：起源、生物学特性、稳态维持和治疗潜力。
Clin Transl Med. 2024 May;14(5):e1720. doi: 10.1002/ctm2.1720.
5
Irradiation-induced hair graying in mice: an experimental model to evaluate the effectiveness of interventions targeting oxidative stress, DNA damage prevention, and cellular senescence.辐照诱导的小鼠毛发变白：一种评估针对氧化应激、DNA 损伤预防和细胞衰老干预措施有效性的实验模型。
Geroscience. 2024 Jun;46(3):3105-3122. doi: 10.1007/s11357-023-01042-7. Epub 2024 Jan 6.
6
Systemic and intrinsic functions of ATRX in glial cell fate and CNS myelination in male mice.ATRX 在雄性小鼠胶质细胞命运和中枢神经系统髓鞘形成中的系统性和内在功能。
Nat Commun. 2023 Nov 4;14(1):7090. doi: 10.1038/s41467-023-42752-y.
7
A genome-wide genetic screen uncovers determinants of human pigmentation.一项全基因组遗传筛选揭示了人类肤色的决定因素。
Science. 2023 Aug 11;381(6658):eade6289. doi: 10.1126/science.ade6289.
8
SWI/SNF complexes are required for retinal pigmented epithelium differentiation and for the inhibition of cell proliferation and neural differentiation programs.SWI/SNF 复合物对于视网膜色素上皮细胞的分化，以及对细胞增殖和神经分化程序的抑制是必需的。
Development. 2023 Aug 15;150(16). doi: 10.1242/dev.201488. Epub 2023 Aug 21.
9
Sox9 regulates melanocytic fate decision of adult hair follicle stem cells.Sox9调控成年毛囊干细胞的黑素细胞命运决定。
iScience. 2023 May 19;26(6):106919. doi: 10.1016/j.isci.2023.106919. eCollection 2023 Jun 16.
10
BMI1 is required for melanocyte stem cell maintenance and hair pigmentation.BMI1 对于黑素细胞干细胞的维持和毛发色素沉着是必需的。
Pigment Cell Melanoma Res. 2023 Sep;36(5):399-406. doi: 10.1111/pcmr.13088. Epub 2023 May 3.
Sox10 通过多个调节靶基因控制 B16F10 黑色素瘤细胞的迁移。
PLoS One. 2012;7(2):e31477. doi: 10.1371/journal.pone.0031477. Epub 2012 Feb 21.
4
Novel Tfap2-mediated control of soxE expression facilitated the evolutionary emergence of the neural crest.新型 Tfap2 介导的 soxE 表达调控促进了神经嵴的演化出现。
Development. 2012 Feb;139(4):720-30. doi: 10.1242/dev.071308. Epub 2012 Jan 12.
5
An iterative genetic and dynamical modelling approach identifies novel features of the gene regulatory network underlying melanocyte development.一种迭代遗传和动力学建模方法确定了黑素细胞发育相关基因调控网络的新特征。
PLoS Genet. 2011 Sep;7(9):e1002265. doi: 10.1371/journal.pgen.1002265. Epub 2011 Sep 1.
6
Functional characterization of melanocyte stem cells in hair follicles.毛囊黑素干细胞的功能特征。
J Invest Dermatol. 2011 Dec;131(12):2358-67. doi: 10.1038/jid.2011.195. Epub 2011 Jul 14.
7
Coordinated activation of Wnt in epithelial and melanocyte stem cells initiates pigmented hair regeneration.Wnt 在上皮和黑素细胞干细胞中的协调激活启动有色素的毛发再生。
Cell. 2011 Jun 10;145(6):941-955. doi: 10.1016/j.cell.2011.05.004.
8
Protective effect of Kit signaling for melanocyte stem cells against radiation-induced genotoxic stress.Kit 信号对黑素细胞干细胞抵御辐射诱导遗传毒性应激的保护作用。
J Invest Dermatol. 2011 Sep;131(9):1906-15. doi: 10.1038/jid.2011.148. Epub 2011 Jun 2.
9
Neural crest cells retain their capability for multipotential differentiation even after lineage-restricted stages.神经嵴细胞在谱系限制阶段后仍然保留其多能分化的能力。
Dev Dyn. 2011 Jul;240(7):1681-93. doi: 10.1002/dvdy.22658. Epub 2011 May 18.
10
Isolation and live imaging of enteric progenitors based on Sox10-Histone2BVenus transgene expression.基于Sox10-组蛋白2B维纳斯转基因表达的肠道祖细胞的分离与实时成像
Genesis. 2011 Jul;49(7):599-618. doi: 10.1002/dvg.20748. Epub 2011 Jun 21.