Axon J E, Carrick J B, Barton M D, Collins N M, Russell C M, Kiehne J, Coombs G
Scone Equine Hospital, Scone, New South Wales 2337, Australia.
Aust Vet J. 2011 Jun;89(6):221-5. doi: 10.1111/j.1751-0813.2011.00711.x.
To evaluate if methicillin-resistant Staphylococcus aureus (MRSA) is present in the horse population in Australia.
A two-part retrospective study of laboratory submissions of microbial culture results from horses.
Part A: medical records of 216 horses that had MRSA screening performed on nasal swabs collected over a 30-day period at admission to the Scone Equine Hospital Clovelly Intensive Care Unit were retrieved. Part B: laboratory records from 2004 to 2009 of culture submissions to the Scone Veterinary Laboratory were reviewed and cultures that grew MRSA were identified. The MRSA isolates from Parts A and B were genotyped over an 18-month period.
MRSA screening of 216 horses identified eight (3.7%) positive samples. MRSA was isolated from cultures of 80 (0.002%) clinical bacteriology samples over a 6-year period. Genotypic analysis was performed on 36 isolates. All MRSA characterised had the same pulse field gel electrophoresis pattern (type 1), with eight closely related subtypes identified (subtypes A-F and H) and 66% of isolates classified as subtype D, which multilocus sequence and staphylococcal cassette chromosome mec typing analysis identified as ST612-MRSA-IVa, a clonal complex (CC) 8 S. aureus strain. Antimicrobial resistance to more than two classes of antimicrobials was common.
MRSA was present in a population of horses in Australia. Genotypic analysis of the isolates identified the MRSA strain as CC8 S. aureus. Further research needs to be undertaken to evaluate MRSA infection and colonisation of horses and personnel in Australia.
评估澳大利亚马群中是否存在耐甲氧西林金黄色葡萄球菌（MRSA）。
对马匹微生物培养结果的实验室送检记录进行两部分的回顾性研究。
A部分：检索在斯昆马医院克洛弗利重症监护病房入院时30天内采集的216匹马鼻拭子进行MRSA筛查的病历。B部分：回顾2004年至2009年提交给斯昆兽医实验室的培养记录，并鉴定出培养出MRSA的培养物。在18个月期间对A部分和B部分的MRSA分离株进行基因分型。
对216匹马进行MRSA筛查，发现8份（3.7%）阳性样本。在6年期间，从80份（0.002%）临床细菌学样本培养物中分离出MRSA。对36株分离株进行了基因分型。所有鉴定出的MRSA具有相同的脉冲场凝胶电泳图谱（1型），鉴定出8个密切相关的亚型（A - F和H亚型），66%的分离株归类为D亚型，多位点序列和葡萄球菌盒式染色体mec分型分析确定为ST612 - MRSA - IVa，一种克隆复合体（CC）8金黄色葡萄球菌菌株。对两类以上抗菌药物耐药很常见。
澳大利亚马群中存在MRSA。对分离株的基因分型确定该MRSA菌株为CC8金黄色葡萄球菌。需要进一步开展研究以评估澳大利亚马匹和人员的MRSA感染及定植情况。
