Postgraduate Programme in Health Science, State University of Maringá, Paraná, Brazil.
Clin Microbiol Infect. 2011 Dec;17(12):1792-7. doi: 10.1111/j.1469-0691.2011.03508.x. Epub 2011 May 20.
The microscopic observation drug susceptibility assay (MODS) was evaluated to determine susceptibility to pyrazinamide in Mycobacterium tuberculosis, and compared with the broth microdilution method (BMM), absolute concentration method (ACM), and pyrazinamidase (PZase) determination. We tested 34 M. tuberculosis clinical isolates (24 sensitive and eight resistant to pyrazinamide) and the control strains M. tuberculosis H37Rv (ATCC 27294) and Mycobacterium bovis AN5. The MODS, BMM, ACM and PZase determination provided results in average times of 6, 18, 28 and 7 days, respectively. All methods showed excellent sensitivity and specificity (p <0.05). Of the methods studied, the MODS proved to be faster, efficient, inexpensive, and easy to perform. However, additional studies evaluating the MODS in differentiating pyrazinamide-resistant and pyrazinamide-susceptible M. tuberculosis must be conducted with a larger number of clinical isolates.
微量观察药物敏感性检测法（MODS）用于检测结核分枝杆菌对吡嗪酰胺的敏感性，并与肉汤微量稀释法（BMM）、绝对浓度法（ACM）和吡嗪酰胺酶（PZase）测定进行比较。我们检测了 34 株结核分枝杆菌临床分离株（24 株对吡嗪酰胺敏感，8 株耐药）和对照菌株结核分枝杆菌 H37Rv（ATCC 27294）和牛分枝杆菌 AN5。MODS、BMM、ACM 和 PZase 测定的平均时间分别为 6、18、28 和 7 天。所有方法均显示出良好的敏感性和特异性（p<0.05）。在所研究的方法中，MODS 被证明更快、高效、廉价且易于操作。然而，必须用更多的临床分离株进行评估 MODS 区分吡嗪酰胺耐药和吡嗪酰胺敏感结核分枝杆菌的能力的进一步研究。
