用于快速鉴定耐吡嗪酰胺结核分枝杆菌菌株中pncA基因突变的线性探针检测法的开发与评估
Development and evaluation of a line probe assay for rapid identification of pncA mutations in pyrazinamide-resistant mycobacterium tuberculosis strains.
作者信息
Sekiguchi Jun-Ichiro, Nakamura Tomohiko, Miyoshi-Akiyama Tohru, Kirikae Fumiko, Kobayashi Intetsu, Augustynowicz-Kopec Ewa, Zwolska Zofia, Morita Koji, Suetake Toshinori, Yoshida Hiroshi, Kato Seiya, Mori Toru, Kirikae Teruo
机构信息
Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku, Tokyo 162-8655, Japan.
出版信息
J Clin Microbiol. 2007 Sep;45(9):2802-7. doi: 10.1128/JCM.00352-07. Epub 2007 Jun 27.
Abstract
Resistance of Mycobacterium tuberculosis to pyrazinamide (PZA) derives mainly from mutations in the pncA gene. We developed a reverse hybridization-based line probe assay with oligonucleotide probes designed to detect mutations in pncA. The detection of PZA resistance was evaluated in 258 clinical isolates of M. tuberculosis. The sensitivity and specificity of PZA resistance obtained by this new assay were both 100%, consistent with the results of conventional PZA susceptibility testing. This assay can be used with sputa from tuberculosis patients. It appears to be reliable and widely applicable and, given its simplicity and rapid performance, will be a valuable tool for diagnostic use.
摘要
结核分枝杆菌对吡嗪酰胺（PZA）的耐药性主要源于pncA基因突变。我们开发了一种基于反向杂交的线性探针检测法，使用设计用于检测pncA基因突变的寡核苷酸探针。在258株结核分枝杆菌临床分离株中评估了对PZA耐药性的检测。通过这种新检测法获得的PZA耐药性的敏感性和特异性均为100%，与传统PZA药敏试验结果一致。该检测法可用于结核病患者的痰液检测。它似乎可靠且广泛适用，鉴于其操作简单且快速，将成为诊断应用的有价值工具。
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