Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
Clin Microbiol Infect. 2011 Oct;17(10):1507-13. doi: 10.1111/j.1469-0691.2011.03534.x. Epub 2011 May 20.
Staphylococcus aureus isolates from developed countries have been extensively analyzed with respect to their virulence patterns and clonal relatedness but there is only sparse information on the molecular diversity of S. aureus isolates from Africa. In particular, little is known about S. aureus isolates from asymptomatic carriers compared with isolates causing infections. From 2008 to 2010, we prospectively collected S. aureus isolates from asymptomatic carriers and infections in Lambaréné, Gabon, Central Africa. For these isolates, we determined major virulence factors, and performed multilocus sequence typing (MLST) and spa typing. Among 163 S. aureus isolates from asymptomatic carriers, we found the MLST clonal complexes (CCs) 5, 6, 7, 8, 9, 15, 25, 30, 45, 88, 101, 121 and 152; 3.7% were methicillin-resistant (MRSA). The clinical isolates were associated with CCs 5, 8, 9, 15, 88, 121 and 152; 11% were MRSA. Sequence types 1 and 88 were significantly associated with infection and sequence type 508 was associated with carriage. Remarkably, there was a high prevalence of Panton-Valentine leukocidin (PVL) -encoding genes both in disease-related isolates (57.4%) and in carrier isolates (40.5%). We found differences in the clonal structure and virulence pattern of Gabonese S. aureus isolates from asymptomatic carriers and infections. Of note, S. aureus isolates from Gabon show a very high prevalence of PVL-encoding genes, which exceeds the rates observed for developed countries.
从发达国家分离的金黄色葡萄球菌已广泛分析其毒力模式和克隆相关性，但关于非洲金黄色葡萄球菌分离株的分子多样性的信息却很少。特别是，与引起感染的分离株相比，人们对无症状携带者的金黄色葡萄球菌分离株知之甚少。2008 年至 2010 年，我们在中非加蓬的兰巴雷内前瞻性地收集了无症状携带者和感染患者的金黄色葡萄球菌分离株。对于这些分离株，我们确定了主要的毒力因子，并进行了多位点序列分型（MLST）和 spa 分型。在 163 株来自无症状携带者的金黄色葡萄球菌分离株中，我们发现了 MLST 克隆复合体（CC）5、6、7、8、9、15、25、30、45、88、101、121 和 152；3.7%为耐甲氧西林金黄色葡萄球菌（MRSA）。临床分离株与 CC5、8、9、15、88、121 和 152 相关；11%为 MRSA。ST1 和 88 与感染显著相关，而 ST508 与携带有关。值得注意的是，疾病相关分离株（57.4%）和携带分离株（40.5%）中均存在高流行的杀白细胞素（PVL）编码基因。我们发现了加蓬无症状携带者和感染患者金黄色葡萄球菌分离株的克隆结构和毒力模式存在差异。值得注意的是，来自加蓬的金黄色葡萄球菌分离株的 PVL 编码基因的流行率非常高，超过了发达国家的观察率。
