Özdemir Fatma
Faculty of Arts and Sciences, Department of Biology, Bolu Abant Izzet Baysal University, 14030 Gölköy, Bolu, Turkey.
Biomed Res Int. 2022 Sep 14;2022:6035987. doi: 10.1155/2022/6035987. eCollection 2022.
With a high capacity to acquire antimicrobial resistance, is an important pathogen causing severe infections in animals and humans. A total of 50 isolates from retail ground beef, chicken meat, and fish were characterized by antimicrobial resistance profiling, staphylococcal protein A gene () typing, and multilocus sequence typing (MLST). The broth microdilution test results showed that all isolates were resistant to penicillin and sulphamethoxazole but had varying resistance rates to tetracycline (24%), erythromycin (4%), gentamicin (2%), ciprofloxacin (2%), trimethoprim (2%), and chloramphenicol (0%). The and genes were detected in 100% of the isolates followed by (94%), (92%), (80%), (60%), (26%), (2%), (2%), (0%), and (0%). Moreover, 26% of the isolates were multidrug-resistant, with five or more resistance genes. The typing analysis revealed 22 types, with t091 (16%), t1677 (8%), and t14538 (8%) being the most common, and one new type, t19851, was uncovered. MLST identified seven sequence types (STs), with ST7 (40%), ST15 (20%), and ST199 (13%) being the most common, and two STs (ST7435 and ST7436) were newly identified. In this study, isolated from raw meat showed multidrug resistance and different clones associated with human infections. As a result, foods of animal origin may act as potential vehicles for transmission of multidrug-resistant isolates, and the dissemination of potentially pathogenic clonal types, posing a health risk to humans.
由于具有很强的获得抗微生物耐药性的能力，[具体细菌名称未给出]是一种在动物和人类中引起严重感染的重要病原体。对从零售绞碎牛肉、鸡肉和鱼肉中分离出的总共50株[具体细菌名称未给出]菌株进行了抗微生物耐药性分析、葡萄球菌蛋白A基因（spa）分型和多位点序列分型（MLST）。肉汤微量稀释试验结果表明，所有分离株均对青霉素和磺胺甲恶唑耐药，但对四环素（24%）、红霉素（4%）、庆大霉素（2%）、环丙沙星（2%）、甲氧苄啶（2%）和氯霉素（0%）的耐药率各不相同。100%的分离株检测到spa和femA基因，其次是cna（94%）、clfA（92%）、sdrC（80%）、sdrD（60%）、sbi（26%）、ebpS（2%）、hlb（2%）、eta（0%）和etb（0%）。此外，26%的分离株对五种或更多耐药基因呈多重耐药。spa分型分析揭示了22种spa类型，其中t091（16%）、t1677（8%）和t14538（8%）最为常见，并且发现了一种新的spa类型t19851。MLST鉴定出七种序列类型（STs），其中ST7（40%）、ST15（20%）和ST199（13%）最为常见，并且新鉴定出两种STs（ST7435和ST7436）。在本研究中，从生肉中分离出的[具体细菌名称未给出]表现出多重耐药性以及与人类感染相关的不同克隆。因此，动物源性食品可能成为多重耐药[具体细菌名称未给出]分离株传播的潜在载体，以及潜在致病克隆类型传播的载体，对人类健康构成风险。
