Owais Dima, Al-Groom Rania M, AlRamadneh Tareq Nayef, Alsawalha Laila, Khan Mohd Sajjad Ahmad, Yousef Omar H, Burjaq Shereen Z
Department of Allied Medical Sciences, Al-Balqa Applied University, Salt, Jordan.
Department of Medical Laboratory Science, Faculty of Allied Medical Sciences, Zarqa University, Zarqa, Jordan.
Iran J Microbiol. 2024 Aug;16(4):450-458. doi: 10.18502/ijm.v16i4.16303.
Microbial biofilm is characterized by the irreversible attachment of planktonic cells to a surface and is usually associated with high antimicrobial resistance with worsening the wound healing. The objective of the study was to determine the prevalence of in diabetic foot ulcers (DFUs) of diabetic patients and to investigate antibiotic susceptibility patterns of these isolates. In addition to screen biofilm forming ability of isolated
A total of 112 non-healing wound swabs of diabetic foot patients were collected and cultured on different culture media to identify and characterize 98 isolates. The isolates were examined for their antibiotic susceptibility to different antimicrobial agents. Furthermore, isolates were evaluated for their biofilm production capability using the Tissue Culture Plate Method (TPC). The level of gene expression was determined by RT-PCR.
The results of this study showed that these non-healing wounds yield positive cultures, with an average of 1.67 organisms per sample. The isolates showed highest resistance against oxacillin (95.2%) and lowest resistance against linezolid (3.7%). All isolates were biofilm producers and a significant association with the gene expression level was recorded.
This study showed that isolates have a great ability to produce biofilms that are associated with the chronicity of wounds in diabetic patients. Routine screening for biofilm formers in chronic wounds and their antibiotic susceptibility testing will help in early treatment and prevent any other complications.
微生物生物膜的特征是浮游细胞不可逆地附着于表面，通常与高抗菌耐药性相关，会使伤口愈合恶化。本研究的目的是确定糖尿病患者糖尿病足溃疡（DFU）中[具体微生物名称未给出]的患病率，并调查这些分离株的抗生素敏感性模式。此外，筛选分离株的生物膜形成能力。
收集了112例糖尿病足患者的不愈合伤口拭子，在不同培养基上进行培养，以鉴定和表征98株分离株。检测这些分离株对不同抗菌剂的抗生素敏感性。此外，使用组织培养板法（TPC）评估分离株的生物膜产生能力。通过逆转录聚合酶链反应（RT-PCR）测定[相关基因名称未给出]基因表达水平。
本研究结果表明，这些不愈合伤口培养结果呈阳性，每个样本平均有1.67种微生物。分离株对苯唑西林的耐药性最高（95.2%），对利奈唑胺的耐药性最低（3.7%）。所有分离株均为生物膜产生菌，且记录到与[相关基因名称未给出]基因表达水平有显著关联。
本研究表明，[具体微生物名称未给出]分离株具有很强的生物膜产生能力，这与糖尿病患者伤口的慢性化有关。对慢性伤口中的生物膜形成菌进行常规筛查及其抗生素敏感性测试将有助于早期治疗并预防任何其他并发症。
