• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

抑制脑内白细胞介素-6 转导信号有助于从脂多糖诱导的疾病行为中恢复。

Inhibition of interleukin-6 trans-signaling in the brain facilitates recovery from lipopolysaccharide-induced sickness behavior.

机构信息

Laboratory of Integrative Immunology and Behavior, Animal Science Department, University of Illinois at Urbana-Champaign, Urbana, 7 Animal Sciences Lab 1207 W, Gregory Dr, Urbana, IL 61801, USA.

出版信息

J Neuroinflammation. 2011 May 19;8:54. doi: 10.1186/1742-2094-8-54.

DOI:10.1186/1742-2094-8-54
PMID:21595956
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3113341/
Abstract

BACKGROUND

Interleukin (IL)-6 is produced in the brain during peripheral infection and plays an important but poorly understood role in sickness behavior. Therefore, this study investigated the capacity of soluble gp130 (sgp130), a natural inhibitor of the IL-6 trans-signaling pathway to regulate IL-6 production in microglia and neurons in vitro and its effects on lipopolysaccharide (LPS)-induced sickness behavior in vivo.

METHODS

A murine microglia (BV.2) and neuronal cell line (Neuro.2A) were used to study the effects of stimulating and inhibiting the IL-6 signaling pathway in vitro. In vivo, adult (3-6 mo) BALB/c mice received an intracerebroventricular (ICV) injection of sgp130 followed by an intraperitoneal (i.p.) injection of LPS, and sickness behavior and markers of neuroinflammation were measured.

RESULTS

Soluble gp130 attenuated IL-6- and LPS-stimulated IL-6 receptor (IL-6R) activation along with IL-6 protein release in both microglial (BV.2) and neuronal (Neuro.2A) cell types in vitro. Moreover, in vivo experiments showed that sgp130 facilitated recovery from LPS-induced sickness, and this sgp130-associated recovery was paralleled by reduced IL-6 receptor signaling, mRNA, and protein levels of IL-6 in the hippocampus.

CONCLUSIONS

Taken together, the results show that sgp130 may exert an anti-inflammatory effect on microglia and neurons by inhibiting IL-6 binding. These data indicate that sgp130 inhibits the LPS-induced IL-6 trans-signal and show IL-6 and its receptor are involved in maintaining sickness behavior.

摘要

背景

白细胞介素 (IL)-6 在周围感染时在大脑中产生，并在疾病行为中发挥重要但尚未充分理解的作用。因此，本研究调查了可溶性 gp130 (sgp130) 的能力，sgp130 是 IL-6 转信号通路的天然抑制剂，可调节体外小胶质细胞和神经元中的 IL-6 产生及其对体内脂多糖 (LPS) 诱导的疾病行为的影响。

方法

使用鼠小胶质细胞 (BV.2) 和神经元细胞系 (Neuro.2A) 研究体外刺激和抑制 IL-6 信号通路的影响。在体内，成年 (3-6 个月) BALB/c 小鼠接受脑室内 (ICV) 注射 sgp130 后腹腔内 (i.p.) 注射 LPS，并测量疾病行为和神经炎症标志物。

结果

可溶性 gp130 减弱了体外小胶质细胞 (BV.2) 和神经元 (Neuro.2A) 细胞类型中 IL-6 和 LPS 刺激的 IL-6 受体 (IL-6R) 激活以及 IL-6 蛋白释放。此外，体内实验表明 sgp130 促进了 LPS 诱导的疾病的恢复，sgp130 相关的恢复与海马体中 IL-6 受体信号、IL-6 mRNA 和蛋白水平降低有关。

结论

总之，这些结果表明 sgp130 可能通过抑制 IL-6 结合对小胶质细胞和神经元发挥抗炎作用。这些数据表明 sgp130 抑制 LPS 诱导的 IL-6 转信号，并表明 IL-6 及其受体参与维持疾病行为。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3186/3113341/e598ff46bfa3/1742-2094-8-54-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3186/3113341/1bed07f5dde5/1742-2094-8-54-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3186/3113341/220c8634d60c/1742-2094-8-54-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3186/3113341/30dad098f9c7/1742-2094-8-54-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3186/3113341/4a227bc91a13/1742-2094-8-54-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3186/3113341/a9e3a4750f34/1742-2094-8-54-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3186/3113341/e598ff46bfa3/1742-2094-8-54-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3186/3113341/1bed07f5dde5/1742-2094-8-54-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3186/3113341/220c8634d60c/1742-2094-8-54-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3186/3113341/30dad098f9c7/1742-2094-8-54-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3186/3113341/4a227bc91a13/1742-2094-8-54-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3186/3113341/a9e3a4750f34/1742-2094-8-54-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3186/3113341/e598ff46bfa3/1742-2094-8-54-6.jpg

相似文献

1
Inhibition of interleukin-6 trans-signaling in the brain facilitates recovery from lipopolysaccharide-induced sickness behavior.抑制脑内白细胞介素-6 转导信号有助于从脂多糖诱导的疾病行为中恢复。
J Neuroinflammation. 2011 May 19;8:54. doi: 10.1186/1742-2094-8-54.
2
Central inhibition of interleukin-6 trans-signaling during peripheral infection reduced neuroinflammation and sickness in aged mice.外周感染时白细胞介素-6 转导信号的中枢抑制可减少老年小鼠的神经炎症和疾病。
Brain Behav Immun. 2013 May;30:66-72. doi: 10.1016/j.bbi.2013.01.002. Epub 2013 Jan 23.
3
Minocycline attenuates lipopolysaccharide (LPS)-induced neuroinflammation, sickness behavior, and anhedonia.米诺环素可减轻脂多糖（LPS）诱导的神经炎症、疾病行为和快感缺失。
J Neuroinflammation. 2008 May 13;5:15. doi: 10.1186/1742-2094-5-15.
4
Interleukin-6 trans-signaling in the senescent mouse brain is involved in infection-related deficits in contextual fear conditioning.白细胞介素-6 转导信号在衰老小鼠大脑中与感染相关的情景性恐惧条件反射缺陷有关。
Brain Behav Immun. 2012 Jul;26(5):732-8. doi: 10.1016/j.bbi.2011.10.008. Epub 2011 Oct 29.
5
Different Soluble Forms of the Interleukin-6 Family Signal Transducer gp130 Fine-tune the Blockade of Interleukin-6 Trans-signaling.白细胞介素-6家族信号转导子gp130的不同可溶性形式微调白细胞介素-6反式信号传导的阻断作用。
J Biol Chem. 2016 Jul 29;291(31):16186-96. doi: 10.1074/jbc.M116.718551. Epub 2016 May 23.
6
The balance of interleukin (IL)-6, IL-6·soluble IL-6 receptor (sIL-6R), and IL-6·sIL-6R·sgp130 complexes allows simultaneous classic and trans-signaling.白细胞介素 (IL)-6、IL-6·可溶性 IL-6 受体 (sIL-6R) 和 IL-6·sIL-6R·sgp130 复合物的平衡允许经典信号和转导信号同时发生。
J Biol Chem. 2018 May 4;293(18):6762-6775. doi: 10.1074/jbc.RA117.001163. Epub 2018 Mar 20.
7
Inhibition of classic signaling is a novel function of soluble glycoprotein 130 (sgp130), which is controlled by the ratio of interleukin 6 and soluble interleukin 6 receptor.抑制经典信号通路是可溶性糖蛋白 130（sgp130）的一个新功能，其受到白细胞介素 6 和可溶性白细胞介素 6 受体比值的控制。
J Biol Chem. 2011 Dec 16;286(50):42959-70. doi: 10.1074/jbc.M111.295758. Epub 2011 Oct 11.
8
Alternative intronic polyadenylation generates the interleukin-6 trans-signaling inhibitor sgp130-E10.可变内含子聚腺苷酸化产生白细胞介素-6反式信号传导抑制剂sgp130-E10。
J Biol Chem. 2014 Aug 8;289(32):22140-50. doi: 10.1074/jbc.M114.560938. Epub 2014 Jun 27.
9
IL-6 trans-signaling system in intra-amniotic inflammation, preterm birth, and preterm premature rupture of the membranes.IL-6 转导信号系统在羊膜内炎症、早产及胎膜早破中的作用。
J Immunol. 2011 Mar 1;186(5):3226-36. doi: 10.4049/jimmunol.1003587. Epub 2011 Jan 31.
10
Central Activation of Alpha7 Nicotinic Signaling Attenuates LPS-Induced Neuroinflammation and Sickness Behavior in Adult but Not in Aged Animals.α7 型烟碱型乙酰胆碱受体中央激活减轻 LPS 诱导的成年动物而非老年动物的神经炎症和疾病行为。
Molecules. 2021 Apr 7;26(8):2107. doi: 10.3390/molecules26082107.

引用本文的文献

1
Exercise-mediated IL-6 downstream effects modulate brain pathology-can exercise training protocols influence the downstream effects?运动介导的白细胞介素-6下游效应调节脑病理学——运动训练方案能否影响下游效应？
Front Neurol. 2025 Aug 6;16:1639427. doi: 10.3389/fneur.2025.1639427. eCollection 2025.
2
Inhibition of IL-6 trans-signaling promotes post-stroke functional recovery in a sex and dose-dependent manner.抑制IL-6转信号传导以性别和剂量依赖性方式促进中风后功能恢复。
J Neuroinflammation. 2025 Feb 26;22(1):52. doi: 10.1186/s12974-025-03365-y.
3
Effect of Residue Feed on Immunity, Antioxidant Capacity, and Transcriptome in Chickens: A Study on Sanhuang Chickens.

本文引用的文献

1
Luteolin inhibits microglia and alters hippocampal-dependent spatial working memory in aged mice.木犀草素抑制老年小鼠的小胶质细胞并改变海马依赖性空间工作记忆。
J Nutr. 2010 Oct;140(10):1892-8. doi: 10.3945/jn.110.123273. Epub 2010 Aug 4.
2
GSK3 influences social preference and anxiety-related behaviors during social interaction in a mouse model of fragile X syndrome and autism.GSK3 影响脆性 X 综合征和自闭症小鼠模型社交互动中的社会偏好和焦虑相关行为。
PLoS One. 2010 Mar 16;5(3):e9706. doi: 10.1371/journal.pone.0009706.
3
Consuming a diet supplemented with resveratrol reduced infection-related neuroinflammation and deficits in working memory in aged mice.
残留饲料对鸡的免疫力、抗氧化能力和转录组的影响：以三黄鸡为例的研究
Animals (Basel). 2025 Jan 3;15(1):94. doi: 10.3390/ani15010094.
4
The influence of sex on neuroimmune communication, pain, and physiology.性别对神经免疫通讯、疼痛和生理学的影响。
Biol Sex Differ. 2024 Oct 22;15(1):82. doi: 10.1186/s13293-024-00660-w.
5
Role of metabolic dysfunction and inflammation along the liver-brain axis in animal models with obesity-induced neurodegeneration.代谢功能障碍和炎症在肝脏-脑轴上在肥胖诱导的神经退行性变动物模型中的作用。
Neural Regen Res. 2025 Apr 1;20(4):1069-1076. doi: 10.4103/NRR.NRR-D-23-01770. Epub 2024 May 17.
6
Search in the Periphery for Potential Inflammatory Biomarkers of Dementia with Lewy Bodies and Alzheimer's Disease.在周围寻找路易体痴呆症和阿尔茨海默病潜在的炎症生物标志物。
J Alzheimers Dis. 2024;99(3):1147-1158. doi: 10.3233/JAD-231471.
7
Sex differences in offspring risk and resilience following 11β-hydroxylase antagonism in a rodent model of maternal immune activation.母体免疫激活的啮齿动物模型中 11β-羟化酶拮抗作用后后代风险和弹性的性别差异。
Neuropsychopharmacology. 2024 Jun;49(7):1078-1090. doi: 10.1038/s41386-023-01771-5. Epub 2023 Nov 25.
8
Circadian Rhythm, Sleep, and Immune Response and the Fight against COVID-19.昼夜节律、睡眠、免疫反应与抗击新冠疫情
Oman Med J. 2023 Mar 31;38(2):e477. doi: 10.5001/omj.2023.38. eCollection 2023 Mar.
9
Anti-inflammatory effects of β-FNA are sex-dependent in a pre-clinical model of LPS-induced inflammation.在脂多糖诱导的炎症临床前模型中，β-氟去甲肾上腺素的抗炎作用具有性别依赖性。
J Inflamm (Lond). 2023 Jan 25;20(1):4. doi: 10.1186/s12950-023-00328-z.
10
Age and sex drive differential behavioral and neuroimmune phenotypes during postoperative pain.术后疼痛期间年龄和性别驱动的不同行为和神经免疫表型。
Neurobiol Aging. 2023 Mar;123:129-144. doi: 10.1016/j.neurobiolaging.2022.09.008. Epub 2022 Nov 23.
摄入富含白藜芦醇的饮食可减少老年小鼠与感染相关的神经炎症和工作记忆缺陷。
Rejuvenation Res. 2009 Dec;12(6):445-53. doi: 10.1089/rej.2009.0888.
4
Cannabinoid 1 receptor and interleukin-6 receptor together induce integration of protein kinase and transcription factor signaling to trigger neurite outgrowth.大麻素 1 型受体和白细胞介素-6 受体共同诱导蛋白激酶和转录因子信号的整合，从而触发神经突生长。
J Biol Chem. 2010 Jan 8;285(2):1358-70. doi: 10.1074/jbc.M109.049841. Epub 2009 Oct 27.
5
Microglial physiology: unique stimuli, specialized responses.小胶质细胞生理学：独特的刺激，特殊的反应。
Annu Rev Immunol. 2009;27:119-45. doi: 10.1146/annurev.immunol.021908.132528.
6
Central inhibition of interleukin-1beta ameliorates sickness behavior in aged mice.白细胞介素-1β的中枢抑制改善老年小鼠的疾病行为。
Brain Behav Immun. 2009 Mar;23(3):396-401. doi: 10.1016/j.bbi.2008.12.008. Epub 2008 Dec 31.
7
Neuroinflammation associated with aging sensitizes the brain to the effects of infection or stress.与衰老相关的神经炎症会使大脑对感染或压力的影响更加敏感。
Neuroimmunomodulation. 2008;15(4-6):323-30. doi: 10.1159/000156474. Epub 2008 Nov 26.
8
Exacerbated fatigue and motor deficits in interleukin-10-deficient mice after peripheral immune stimulation.外周免疫刺激后白细胞介素-10缺陷小鼠的疲劳和运动功能障碍加剧。
Am J Physiol Regul Integr Comp Physiol. 2008 Oct;295(4):R1109-14. doi: 10.1152/ajpregu.90302.2008. Epub 2008 Jul 23.
9
IL-6 regulates neutrophil trafficking during acute inflammation via STAT3.白细胞介素-6通过信号转导和转录激活因子3在急性炎症期间调节中性粒细胞的迁移。
J Immunol. 2008 Aug 1;181(3):2189-95. doi: 10.4049/jimmunol.181.3.2189.
10
Immune-to-brain signaling: how important are the blood-brain barrier-independent pathways?免疫-脑信号传导：血脑屏障非依赖途径有多重要？
Mol Neurobiol. 2008 Apr-Jun;37(2-3):142-52. doi: 10.1007/s12035-008-8026-z. Epub 2008 Jun 19.