Zhao Yongfei, Zhao Tianzhang, Zi Shunyi, Ou Guoyu, Li Huiying
College of Agriculture and Biological Sciences, Dali University, Dali 671000, China.
Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, College of Pharmacy, Dali University, Dali 671000, China.
Animals (Basel). 2025 Jan 3;15(1):94. doi: 10.3390/ani15010094.
This study investigated the effects of residue (PAR) on the immune function, antioxidant capacity, and transcriptome of Sanhuang chickens. Six hundred 30-day-old Sanhuang chickens were divided into six groups with diets of varying PAR replacement of soybean meal from 0 to 100% in increments of 20%. Samples were taken on day 100. The results indicated that PAR did not significantly affect growth performance ( > 0.05). PAR significantly elevated the levels of IL-1β, IL-2, TNF-α, IgA, IgG, and IgM in both the serum and spleen ( < 0.05). Moreover, it markedly increased the GSH-Px, T-AOC, SOD, and CAT levels in the serum and liver ( < 0.05) and reached optimal levels of immune factors and antioxidant indicators at diet 2. Transcriptomic analysis revealed that substituting PAR for soybean meal downregulated genes associated with immune diseases and infectious disease pathways. Substituting soybean meal with PAR enhanced the resistance of Sanhuang chickens to pathogenic factors and oxidative stress, with no impact on growth performance. The optimal improvement was observed with diet 2.
本研究调查了蛋白替代物（PAR）对三黄鸡免疫功能、抗氧化能力和转录组的影响。600只30日龄的三黄鸡被分为六组，日粮中PAR替代豆粕的比例从0%到100%，以20%的增量递增。在第100天采集样本。结果表明，PAR对生长性能没有显著影响（P>0.05）。PAR显著提高了血清和脾脏中IL-1β、IL-2、TNF-α、IgA、IgG和IgM的水平（P<0.05）。此外，它显著提高了血清和肝脏中GSH-Px、T-AOC、SOD和CAT的水平（P<0.05），并且在日粮2时免疫因子和抗氧化指标达到最佳水平。转录组分析显示，用PAR替代豆粕下调了与免疫疾病和传染病途径相关的基因。用PAR替代豆粕增强了三黄鸡对致病因素和氧化应激的抵抗力，对生长性能没有影响。日粮2观察到最佳改善效果。
