基于铜（I）催化点击化学的电化学阻抗光谱传感器用于抗坏血酸检测。

Electrochemical impedance spectroscopy sensor for ascorbic acid based on copper(I) catalyzed click chemistry.

机构信息

MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China.

出版信息

Biosens Bioelectron. 2011 Jul 15;26(11):4326-30. doi: 10.1016/j.bios.2011.04.029. Epub 2011 Apr 22.

DOI:10.1016/j.bios.2011.04.029

PMID:21596552

Abstract

Copper(I) species can be acquired from the reduction of copper(II) by ascorbic acid (AA) in situ, and which in turn quantitative catalyze the azides and alkynes cycloaddition reaction. In this study, propargyl-functionalized ferrocene (propargyl-functionalized Fc) has been modified on the electrode through reacting with azide terminal modified Au electrode via copper(I) catalyzed azides and alkynes cycloaddition (CuAAC) reaction. The electrochemical impedance spectroscopy (EIS) measurement has been applied to test the electron transfer resistance of the Au electrode before and after click reaction. The changes of the fractional surface coverage (θ) with different AA concentrations are characterized. It is found that the θ value has a linear response to the logarithm of AA concentration in the range of 5.0 pmol/L to 1.0 nmol/L with the detection limits of 2.6 pmol/L. The sensor shows a good stability and selectivity. And it has been successfully applied to the AA detection in the real samples (urine) with satisfactory results.

摘要

铜（I）物种可以通过抗坏血酸（AA）原位还原铜（II）获得，而铜（I）又可以定量催化叠氮化物和炔烃的环加成反应。在这项研究中，通过铜（I）催化的叠氮化物和炔烃环加成（CuAAC）反应，将炔丙基功能化的二茂铁（炔丙基功能化的 Fc）修饰到电极上。通过电化学阻抗谱（EIS）测量，测试了点击反应前后 Au 电极的电子转移电阻。用不同浓度 AA 来表征不同的分数表面覆盖率（θ）的变化。结果表明，在 5.0 pmol/L 至 1.0 nmol/L 的范围内，θ 值与 AA 浓度的对数呈线性响应，检测限为 2.6 pmol/L。该传感器具有良好的稳定性和选择性。并已成功应用于实际样品（尿液）中 AA 的检测，结果令人满意。