一种二铁蛋白自动生成缬氨酸-苯丙氨酸交联物。
A diiron protein autogenerates a valine-phenylalanine cross-link.
机构信息
Department of Biochemistry and Biophysics, 2011 Agriculture and Life Sciences Building, Oregon State University, Corvallis, OR 97331, USA.
出版信息
Science. 2011 May 20;332(6032):929. doi: 10.1126/science.1205687.
Abstract
All known internal covalent cross-links in proteins involve functionalized groups having oxygen, nitrogen, or sulfur atoms present to facilitate their formation. Here, we report a carbon-carbon cross-link between two unfunctionalized side chains. This valine-phenyalanine cross-link, produced in an oxygen-dependent reaction, is generated by its own carboxylate-bridged diiron center and serves to stabilize the metallocenter. This finding opens the door to new types of posttranslational modifications, and it demonstrates new catalytic potential of diiron centers.
摘要
所有已知的蛋白质内共价交联都涉及具有氧、氮或硫原子的官能团，以促进其形成。在这里，我们报告了两个非官能化侧链之间的碳-碳交联。这种缬氨酸-苯丙氨酸交联是在依赖氧的反应中产生的，由其自身的羧酸桥联双铁中心生成，并有助于稳定金属中心。这一发现为新型的翻译后修饰开辟了道路，并展示了双铁中心的新催化潜力。
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