Department of Biochemistry and Biophysics, 2011 Agriculture and Life Sciences Building, Oregon State University, Corvallis, OR 97331, USA.
Science. 2011 May 20;332(6032):929. doi: 10.1126/science.1205687.
All known internal covalent cross-links in proteins involve functionalized groups having oxygen, nitrogen, or sulfur atoms present to facilitate their formation. Here, we report a carbon-carbon cross-link between two unfunctionalized side chains. This valine-phenyalanine cross-link, produced in an oxygen-dependent reaction, is generated by its own carboxylate-bridged diiron center and serves to stabilize the metallocenter. This finding opens the door to new types of posttranslational modifications, and it demonstrates new catalytic potential of diiron centers.
所有已知的蛋白质内共价交联都涉及具有氧、氮或硫原子的官能团，以促进其形成。在这里，我们报告了两个非官能化侧链之间的碳-碳交联。这种缬氨酸-苯丙氨酸交联是在依赖氧的反应中产生的，由其自身的羧酸桥联双铁中心生成，并有助于稳定金属中心。这一发现为新型的翻译后修饰开辟了道路，并展示了双铁中心的新催化潜力。
