Institute for Agricultural and Fisheries Research, Technology and Food Science Unit, Brusselsesteenweg 370, 9090 Melle, Belgium.
Poult Sci. 2011 Jun;90(6):1185-90. doi: 10.3382/ps.2010-01178.
The present study evaluated Escherichia coli, Enterococcus faecalis, and Enterococcus hirae as potential indicator organisms for the possible Salmonella Enteritidis (SE) presence in layer farms after cleaning and disinfection by comparing their susceptibility to disinfection. A quantitative suspension disinfection test according to European Standard EN1656 was performed using disinfection products CID20 and Virocid (both from CID Lines, Ieper, Belgium). In a preliminary test, the sensitivity to both disinfection products was compared between ATCC strains of SE, E. coli, En. faecalis, and En. hirae. The sensitivity of SE to disinfection was most comparable to that of E. coli. A second disinfection test compared the elimination of E. coli to SE ATCC strains as well as field strains. Results showed no significant effect regarding the strain (P > 0.05 for CID20 and Virocid), meaning that no difference was detected in sensitivity toward disinfection. When comparing the sensitivity in general at species level for all concentrations of disinfectant used, no significant difference was found between E. coli and SE in sensitivity to Virocid (P > 0.05). In conclusion, because of its similar response to disinfection in a suspension disinfection test, E. coli could be used as an indicator for possible Salmonella presence after cleaning and disinfection.
本研究通过比较大肠杆菌、粪肠球菌和屎肠球菌对消毒的敏感性，评估其作为清洁消毒后鸡场中可能存在肠炎沙门氏菌（SE）的潜在指示生物。采用欧洲标准 EN1656 进行了定量悬浮消毒试验，使用了消毒产品 CID20 和 Virocid（均来自比利时伊珀尔的 CID Lines）。在初步试验中，比较了 SE、大肠杆菌、粪肠球菌和屎肠球菌的 ATCC 株对两种消毒产品的敏感性。SE 对消毒的敏感性与大肠杆菌最相似。第二次消毒试验比较了大肠杆菌和 SE ATCC 株以及田间株的消除情况。结果显示，菌株之间没有显著差异（CID20 和 Virocid 的 P 值均大于 0.05），这意味着消毒的敏感性没有差异。当比较所有使用消毒剂浓度的一般种水平的敏感性时，在 Virocid 对大肠杆菌和 SE 的敏感性方面未发现显著差异（P 值大于 0.05）。总之，由于其在悬浮消毒试验中对消毒的反应相似，大肠杆菌可以用作清洁消毒后可能存在沙门氏菌的指示生物。
