Laboratório de Investigação em Biofilmes Rosário Oliveira, Centre of Biological Engineering, University of Minho, Braga, Portugal.
Biofouling. 2020 Jan;36(1):101-112. doi: 10.1080/08927014.2020.1719077. Epub 2020 Jan 30.
Although disinfection procedures are widely implemented in food environments, bacteria can survive and present increased virulence/resistance. Since little is known about these phenomena regarding biofilms, this study aimed to investigate the effect of chemical disinfection on biofilm-derived cells of Enteritidis. Using a reference strain (NCTC 13349) and a food isolate (350), biofilm susceptibility to benzalkonium chloride (BAC), sodium hypochlorite (SH) and hydrogen peroxide (HP) was evaluated and biofilms were exposed to sub-lethal concentrations of each disinfectant. Biofilm-derived cells were characterized for their biofilm forming ability, antibiotic resistance and expression of virulence-associated genes. Except for a few instances, disinfectant exposure did not alter antibiotic susceptibility. However, SH and HP exposure enhanced the biofilm forming ability of Enteritidis NCTC 13349. After BAC and HP exposure, biofilm-derived cells presented a down-regulation of . Exposure to BAC also revealed an up-regulation of and on Enteritidis NCTC 13349. The results obtained suggest that biofilm-derived cells that survive disinfection may represent an increased health risk.
尽管食品环境中广泛实施了消毒程序，但细菌仍能存活并表现出更高的毒力/耐药性。由于对于生物膜而言，这些现象知之甚少，因此本研究旨在调查化学消毒对肠炎沙门氏菌生物膜衍生细胞的影响。使用参考菌株（NCTC 13349）和食品分离株（350），评估了苯扎氯铵（BAC）、次氯酸钠（SH）和过氧化氢（HP）对生物膜的敏感性，并将生物膜暴露于每种消毒剂的亚致死浓度下。对生物膜衍生细胞的生物膜形成能力、抗生素耐药性和毒力相关基因的表达进行了表征。除了少数情况外，消毒剂暴露并没有改变抗生素敏感性。然而，SH 和 HP 暴露增强了肠炎沙门氏菌 NCTC 13349 的生物膜形成能力。BAC 和 HP 暴露后，生物膜衍生细胞的 表达下调。BAC 暴露还导致 NCTC 13349 中 和 的上调。研究结果表明，存活消毒的生物膜衍生细胞可能代表更高的健康风险。
