Bosworth B T, MacLachlan N J
Department of Microbiology, Pathology, and Parasitology, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
Am J Vet Res. 1990 May;51(5):723-5.
The antiviral activity of recombinant DNA-derived bovine alpha 1(-1) interferon on an established swine testicular cell line and primary testicular cell cultures derived from swine of various ages (2 days, 3 weeks, and 5 weeks) was determined. Bovine interferon induced a dose-dependent increase in 2-5A synthetase in testicular cells, regardless of the source of the cells. Furthermore, interferon inhibited replication of vesicular stomatitis virus to an equivalent extent in all testicular cell cultures. The results indicate that 2-5A synthetase is a reliable marker of interferon activity in swine testicular cell cultures and that the induction of 2-5A synthetase and antiviral effects of recombinant bovine interferon in primary testicular cell cultures are not dependent on the age of the donor animal.
测定了重组DNA衍生的牛α1（-1）干扰素对已建立的猪睾丸细胞系以及来自不同年龄（2天、3周和5周）猪的原代睾丸细胞培养物的抗病毒活性。无论细胞来源如何，牛干扰素均可诱导睾丸细胞中2-5A合成酶呈剂量依赖性增加。此外，干扰素在所有睾丸细胞培养物中对水疱性口炎病毒复制的抑制程度相同。结果表明，2-5A合成酶是猪睾丸细胞培养物中干扰素活性的可靠标志物，并且重组牛干扰素在原代睾丸细胞培养物中对2-5A合成酶的诱导和抗病毒作用不依赖于供体动物的年龄。
