干扰素对两种人绒毛膜癌衍生细胞系的作用。
Effect of interferon on two human choriocarcinoma-derived cell lines.
作者信息
Vandenbussche P, Kuwata T, Verhaegen-Lewalle M, Content J
出版信息
Virology. 1983 Jul 30;128(2):474-9. doi: 10.1016/0042-6822(83)90273-8.
Two cell lines derived from human choriocarcinomas (HCCM-5 and BeWo) are resistant to several biological effects of human interferon such as inhibition of VSV multiplication and inhibition of cell growth, but they develop a normal antiviral activity against EMCV. Nevertheless, in both cell lines, 2-5A synthetase and protein kinase are induced by IFN. 2-5A-dependent endonuclease can be measured by two independent methods and 2-5A itself is detected at least in poly(rI):poly(rC)- and IFN-treated BeWo cells. This is another example of two cell lines that are partially, with respect to the antiviral effect toward VSV, and totally, with respect to the anticellular effect, refractory to IFN treatment, although all the known elements of the 2-5A system are present.
从人绒毛膜癌衍生而来的两种细胞系（HCCM - 5和BeWo）对人干扰素的几种生物学效应具有抗性，如抑制水疱性口炎病毒（VSV）增殖和抑制细胞生长，但它们对脑心肌炎病毒（EMCV）具有正常的抗病毒活性。然而，在这两种细胞系中，2 - 5A合成酶和蛋白激酶可被干扰素诱导。2 - 5A依赖性核酸内切酶可用两种独立方法检测，并且至少在聚（rI）：聚（rC）和干扰素处理的BeWo细胞中可检测到2 - 5A本身。这是另外两个细胞系的例子，尽管2 - 5A系统的所有已知成分都存在，但就对VSV的抗病毒作用而言部分地、就抗细胞作用而言完全地对干扰素治疗具有抗性。
Zotero 插件