Goren T, Kapitkovsky A, Kimchi A, Rubinstein M
Virology. 1983 Oct 30;130(2):273-80. doi: 10.1016/0042-6822(83)90082-x.
Three major subtypes of human interferon-alpha (IFN-alpha), isolated from virus-induced leukocytes, were compared for their antiviral and anticellular activities on one hand, and for their ability to induce (2'-5') oligoadenylate synthetase on the other hand. One subtype, IFN-alpha 1, was found to have low specific antiviral (6.10(6)-5.10(7) units/mg) and anticellular activities when measured on a variety of human cells. A second subtype, exhibiting an unusually high molecular weight (26,000) by SDS-polyacrylamide gel electrophoresis (IFN-alpha 26K), was found to have the highest known specific antiviral (8.10(8)-2.10(9) units/mg) and anticellular activities. Thus, these two subtypes of IFN-alpha differ by a factor of 330 and represent the two extremes in the antiviral scale on human cells. A third subtype, IFN-alpha 2, was tested as well and was found to have intermediate antiviral and anticellular activities. The ability of these three subtypes to induce (2'-5') oligoadenylate synthetase in human cells was then measured. It was found that on a weight basis, the three subtypes were equally effective in inducing the enzyme. Since the level of (2'-5') adenylate oligomers is affected also by the interferon-induced (2'-5') phosphodiesterase, the ability of these subtypes to induce this enzyme was compared as well and was found to be very similar. We therefore conclude that the differences in potency between these IFN-alpha subtypes are not related to their ability to induce (2'-5') oligoadenylate synthetase.
从病毒诱导的白细胞中分离出的三种主要人类α-干扰素（IFN-α）亚型，一方面比较它们的抗病毒和抗细胞活性，另一方面比较它们诱导（2'-5'）寡腺苷酸合成酶的能力。发现其中一种亚型IFN-α1，在多种人类细胞上进行检测时，具有较低的特异性抗病毒活性（6.10⁶ - 5.10⁷单位/毫克）和抗细胞活性。通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - 聚丙烯酰胺凝胶电泳）显示分子量异常高（26,000）的第二种亚型（IFN-α26K），被发现具有已知最高的特异性抗病毒活性（8.10⁸ - 2.10⁹单位/毫克）和抗细胞活性。因此，这两种IFN-α亚型相差330倍，代表了人类细胞抗病毒活性范围的两个极端。还测试了第三种亚型IFN-α2，发现其具有中等的抗病毒和抗细胞活性。然后测量了这三种亚型在人类细胞中诱导（2'-5'）寡腺苷酸合成酶的能力。发现以重量为基础，这三种亚型在诱导该酶方面同样有效。由于（2'-5'）腺苷酸寡聚物的水平也受干扰素诱导的（2'-5'）磷酸二酯酶影响，因此也比较了这些亚型诱导该酶的能力，发现非常相似。我们因此得出结论，这些IFN-α亚型之间效力的差异与其诱导（2'-5'）寡腺苷酸合成酶的能力无关。
