Ogundigie P, Roy G, Kanin G, Goto T, Ito A
HIROSHIMA UNIV,RADIAT BIOL & MED RES INST,DEPT CANC RES,KASUMI 1-2-3,MINAMI KU,HIROSHIMA 734,JAPAN.
Oncol Rep. 1995 Mar;2(2):271-5. doi: 10.3892/or.2.2.271.
To determine the biological effect of biochanin A, miso or NaCl and sexual influence of testosterone on liver tumor induction, male and female BCF1 mice were i.p. injected once with DEN at a dose of 5 mug/g body weight at 15 days of age. In order to shorten the latency of liver tumor occurrence, the whole body of mice were exposed to 2 Gy of Cf-252 fission neutrons at four weeks of age. Three days later, female mice were surgically ovariectomized and given the various doses of testosterone melted into a cholesterol pellet. Male mice were fed on 10 ppm, 20 ppm biochanin A, 10% miso or 2% NaCl supplemented diet for 8 weeks (from 21 to 28 weeks of age) and 4 weeks (from 32 to 36 weeks of age). All mice were sacrificed at 40 weeks of age. Multiplicity of liver tumors was expressed in four different size ranges by <2, 3-5, 6-10 and >10 mm2. Incidence of liver tumors in all experimental groups except in group 1 at 20 weeks were observed at 100%. Average tumor size and multiplicity were smaller at 20 weeks compared to those of 40-week groups. Male groups fed 20 ppm biochanin A and 2% NaCl had an increase in body weight with significant difference from control by p<0.01. Liver weights were more-or-less the same in all groups except an increase was seen in the group of 20 ppm biochanin A (p<0.01). In female groups, both 0.2 mg and 1 mg of testosterone administration resulted in an increase of tumor multiplicities and a decrease of liver weight compared to that of control group with significant differences. In both male and female groups, majority of liver tumor sizes were in the range of 3-5 mm2. Tumor multiplicities and size in less than 2 mm2 in biochanin A groups, 10% miso and 2% NaCl decreased significantly from control group. These findings suggest that 15-20 weeks is the time in which 1-2 mm2 size of liver tumors start to appear. Among others, biochanin A is a component of miso. The potent anti-tumorigenic effect of dietary miso for mouse liver tumorigenesis may be strenghtened by a combination of factors such as the presence of Biochanin A, protease inhibitors and various fermented enzymes.
为了确定鹰嘴豆芽素A、味噌或氯化钠的生物学效应以及睾酮对肝肿瘤诱导的性别影响，15日龄的雄性和雌性BCF1小鼠经腹腔注射一次剂量为5μg/g体重的二乙基亚硝胺（DEN）。为了缩短肝肿瘤发生的潜伏期，4周龄时将小鼠全身暴露于2 Gy的Cf-252裂变中子。三天后，对雌性小鼠进行手术切除卵巢，并给予不同剂量溶解于胆固醇丸剂中的睾酮。雄性小鼠喂食添加10 ppm、20 ppm鹰嘴豆芽素A、10%味噌或2%氯化钠的饲料8周（21至28周龄）和4周（32至36周龄）。所有小鼠在40周龄时处死。肝肿瘤的多发性按<2、3 - 5、6 - 10和>10 mm2四个不同大小范围表示。除第1组在20周时外，所有实验组在20周时肝肿瘤发生率均为100%。与40周龄组相比，20周时平均肿瘤大小和多发性较小。喂食20 ppm鹰嘴豆芽素A和2%氯化钠的雄性组体重增加，与对照组相比差异显著（p<0.01）。除20 ppm鹰嘴豆芽素A组体重增加（p<0.01）外，所有组的肝脏重量大致相同。在雌性组中，与对照组相比，给予0.2 mg和1 mg睾酮均导致肿瘤多发性增加和肝脏重量减轻，差异显著。在雄性和雌性组中，大多数肝肿瘤大小在3 - 5 mm2范围内。鹰嘴豆芽素A组、10%味噌和2%氯化钠组中小于2 mm2的肿瘤多发性和大小与对照组相比显著降低。这些发现表明，15 - 20周是1 - 2 mm2大小的肝肿瘤开始出现的时期。其中，鹰嘴豆芽素A是味噌的一种成分。膳食味噌对小鼠肝肿瘤发生的强大抗肿瘤作用可能因多种因素的组合而增强，如鹰嘴豆芽素A的存在、蛋白酶抑制剂和各种发酵酶。
